Protein Kinase C Delta antibody | E-J01/1C2

Mouse anti Protein Kinase C Delta

Product Type
PrecisionAb Monoclonal
Clone
E-J01/1C2
Isotype
IgG1
Format
Purified
Specificity
Protein Kinase C Delta

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Mouse anti Human protein kinase C delta antibody, clone E-J01/1C2 recognizes protein kinase C delta also known as tyrosine-protein kinase PRKCD.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PRKCD is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed (provided by RefSeq, Jul 2008).

Mouse anti Human protein, clone E-J01/1C2 kinase C delta antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti protein kinase C delta detects a band of approximately 78 kDa in A431 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E.coli-derived recombinant protein corresponding to aa 549-676 of human protein kinase C delta
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q05655
Entrez Gene
PRKCD
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004699 calcium-independent protein kinase C activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007202 activation of phospholipase C activity
GO:0008022 protein C-terminus binding
GO:0008047 enzyme activator activity
GO:0010469 regulation of receptor activity
GO:0016071 mRNA metabolic process
GO:0050821 protein stabilization
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0032091 negative regulation of protein binding
GO:0043560 insulin receptor substrate binding
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0090398 cellular senescence
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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