Protein Kinase C Delta antibody | E-J01/1C2

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Anti Protein Kinase C Delta Antibody, clone E-J01/1C2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Protein Kinase C Delta Antibody, clone E-J01/1C2 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Protein Kinase C Delta Antibody, clone E-J01/1C2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with protein kinase C delta antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 26 second exposure. Arrow points to protein kinase C delta (molecular weight 78 kDa).

Anti Protein Kinase C Delta Antibody, clone E-J01/1C2 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of PRKCD from A431 whole cell lysate. The Input lane is 10% input; the PRKCD-IP lane is the immunoprecipitation with the PRKCD antibody (2.5 μg, **VMA00408**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 μg, MCA928). Western blot analysis was performed using PRKCD antibody (1/1000, **VMA00408**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to PRKCD

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Mouse anti Protein Kinase C Delta

Product Type: PrecisionAb Monoclonal
Clone: E-J01/1C2
Isotype: IgG1
Format: Purified
Specificity: Protein Kinase C Delta

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00408 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human protein kinase C delta antibody, clone E-J01/1C2 recognizes protein kinase C delta also known as tyrosine-protein kinase PRKCD.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PRKCD is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed (provided by RefSeq, Jul 2008).

Mouse anti Human protein, clone E-J01/1C2 kinase C delta antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti protein kinase C delta detects a band of approximately 78 kDa in A431 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E.coli-derived recombinant protein corresponding to aa 549-676 of human protein kinase C delta
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q05655
Entrez Gene: PRKCD
GO Terms: GO:0005886 plasma membrane; GO:0005829 cytosol; GO:0004699 calcium-independent protein kinase C activity; GO:0005783 endoplasmic reticulum; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0006468 protein phosphorylation; GO:0006921 cellular component disassembly involved in apoptosis; View More GO Terms; GO:0007202 activation of phospholipase C activity; GO:0008022 protein C-terminus binding; GO:0008047 enzyme activator activity; GO:0010469 regulation of receptor activity; GO:0016071 mRNA metabolic process; GO:0050821 protein stabilization; GO:0019899 enzyme binding; GO:0030168 platelet activation; GO:0032091 negative regulation of protein binding; GO:0043560 insulin receptor substrate binding; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0090398 cellular senescence