Prostate Specific Membrane Antigen antibody | GCP-04

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Mouse anti Prostate Specific Membrane Antigen

Product Type
Monoclonal Antibody
Clone
GCP-04
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2885
Datasheet
P* WB
MSDS
0.1 mg loader

Mouse anti prostate specific Membrane Antigen antibody, clone GCP-04 recognises amino acids 100-104 of the extracellular domain of prostate specific membrane antigen (PSMA), otherwise known as GCPII (glutamate carboxypeptidase II), a 95-110 kDa type II transmembrane glycoprotein and member of the peptidase M28 family.

Nomenclature for GCPII is dependent upon tissue specificity. PSMA is assigned to GCPII of prostate origin which is up-regulated in prostate cancer and a reliable diagnostic marker. Folate hydrolase (FOLH1) describes GCPII in the intestine and is required for folate uptake, whilst NAALADase refers to GCPII expressed in the central nervous system, which acts to cleave N-acetylaspartylglutamate (NAAG) to release glutamate, implicated in pathological conditions including Alzheimer’s and Huntington’s disease.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fragment corresponding to amino acids 44-750 of human GCPII.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q04609 Related reagents
Entrez Gene
FOLH1 Related reagents
Regulatory
For research purposes only

Applications of Prostate Specific Membrane Antigen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human prostate, kidney or spinal cord.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Prostate Specific Membrane Antigen antibody

  1. Rovenská, M. et al. (2008) Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.
    Prostate. 68 (2): 171-82.
  2. Barinka, C. et al. (2004) Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
    Protein Sci. 13 (6): 1627-35.
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