Prostate Specific Membrane Antigen antibody | GCP-04
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Mouse anti Prostate Specific Membrane Antigen
- Product Type
- Monoclonal Antibody
- Clone
- GCP-04
- Isotype
- IgG1
- Specificity
- Prostate Specific Membrane Antigen
Antibody Quality and Performance Guarantee
| Mouse anti prostate specific Membrane Antigen antibody, clone GCP-04 recognises amino acids 100-104 of the extracellular domain of prostate specific membrane antigen (PSMA), otherwise known as GCPII (glutamate carboxypeptidase II), a 95-110 kDa type II transmembrane glycoprotein and member of the peptidase M28 family. Nomenclature for GCPII is dependent upon tissue specificity. PSMA is assigned to GCPII of prostate origin which is up-regulated in prostate cancer and a reliable diagnostic marker. Folate hydrolase (FOLH1) describes GCPII in the intestine and is required for folate uptake, whilst NAALADase refers to GCPII expressed in the central nervous system, which acts to cleave N-acetylaspartylglutamate (NAAG) to release glutamate, implicated in pathological conditions including Alzheimer’s and Huntington’s disease. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant fragment corresponding to amino acids 44-750 of human GCPII.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
||
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human prostate, kidney or spinal cord.
References for Prostate Specific Membrane Antigen antibody
-
Rovenská, M. et al. (2008) Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.
Prostate. 68 (2): 171-82. -
Barinka, C. et al. (2004) Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
Protein Sci. 13 (6): 1627-35.
- Synonyms
- GCP II
- RRID
- AB_2106316
- UniProt
- Q04609
- Entrez Gene
- FOLH1
MCA2885
148688
170720
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with PROSTATE SPECIFIC specificity
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