Prostate Specific Membrane Antigen antibody | GCP-04
Nomenclature for GCPII is dependent upon tissue specificity. PSMA is assigned to GCPII of prostate origin which is up-regulated in prostate cancer and a reliable diagnostic marker. Folate hydrolase (FOLH1) describes GCPII in the intestine and is required for folate uptake, whilst NAALADase refers to GCPII expressed in the central nervous system, which acts to cleave N-acetylaspartylglutamate (NAAG) to release glutamate, implicated in pathological conditions including Alzheimer’s and Huntington’s disease.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant fragment corresponding to amino acids 44-750 of human GCPII.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
Applications of Prostate Specific Membrane Antigen antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human prostate, kidney or spinal cord.
Secondary Antibodies Available
Product Specific References
References for Prostate Specific Membrane Antigen antibody
Rovenská, M. et al. (2008) Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.
Prostate. 68 (2): 171-82.
Barinka, C. et al. (2004) Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
Protein Sci. 13 (6): 1627-35.
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