Prolyl Hydroxylase 1 antibody | PHD112/G7

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Mouse anti Human Prolyl Hydroxylase 1

Product Type
Monoclonal Antibody
Clone
PHD112/G7
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2888GA P* WB 0.1 mg
Mouse anti Human Prolyl Hydroxylase 1 antibody, clone PHD112/G7 recognizes human prolyl hydroxylase 1 (PHD1), a ~44 kDa enzyme expressed abundantly in all tissues with the highest expression in testis.

Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 1 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

Prolyl hydroxylase 1 might also play a role in the regulation of cell growth.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length human PHD1 recombinantly expressed.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
Q96KS0 Related reagents
Entrez Gene
EGLN2 Related reagents
GO Terms
GO:0001558 regulation of cell growth
GO:0001666 response to hypoxia
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0008198 ferrous iron binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0019826 oxygen sensor activity
GO:0030520 estrogen receptor signaling pathway
GO:0031418 L-ascorbic acid binding
GO:0045732 positive regulation of protein catabolic process
Regulatory
For research purposes only

Applications of Prolyl Hydroxylase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human testis

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Application Based External Images

Western Blotting

Product Specific References

References for Prolyl Hydroxylase 1 antibody

  1. Appelhoff, R.J. et al. (2004) Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.
    J Biol Chem. 279: 38458-65.
  2. Boddy, J.L. et al. (2005) The androgen receptor is significantly associated with vascular endothelial growth factor and hypoxia sensing via hypoxia-inducible factors HIF-1a, HIF-2a, and the prolyl hydroxylases in human prostate cancer.
    Clin Cancer Res. 11: 7658-63.