Prohibitin antibody

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Mouse anti Prohibitin

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00284 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human prohibitin antibody recognizes prohibitin, also known as epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e.

The PHB gene is evolutionarily conserved. Encoded by this gene, prohibitin is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of PHB have been identified. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013).

Mouse anti Human prohibitin antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti prohibitin detects a band of approximately 32 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Purified recombinant fragment of human prohibitin expressed in E. coli

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P35232
Entrez Gene: PHB
GO Terms: GO:0006260 DNA replication; GO:0005887 integral to plasma membrane; GO:0005654 nucleoplasm; GO:0005743 mitochondrial inner membrane; GO:0008285 negative regulation of cell proliferation; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0042981 regulation of apoptosis; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0016575 histone deacetylation; GO:0042826 histone deacetylase binding; GO:0042921 glucocorticoid receptor signaling pathway; GO:0050847 progesterone receptor signaling pathway; GO:0060766 negative regulation of androgen receptor signaling pathway
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Prohibitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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