Prohibitin antibody
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WB ResetMouse anti Prohibitin
- Product Type
- PrecisionAb Monoclonal
- Isotype
- IgG1
- Format
- Purified
- Specificity
- Prohibitin
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| Mouse anti Human prohibitin antibody recognizes prohibitin, also known as epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e. The PHB gene is evolutionarily conserved. Encoded by this gene, prohibitin is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of PHB have been identified. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013). Mouse anti Human prohibitin antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti prohibitin detects a band of approximately 32 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Purified recombinant fragment of human prohibitin expressed in E. coli
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P35232
- Entrez Gene
- PHB
- GO Terms
- GO:0006260 DNA replication
- GO:0005887 integral to plasma membrane
- GO:0005654 nucleoplasm
- GO:0005743 mitochondrial inner membrane
- GO:0008285 negative regulation of cell proliferation
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0042981 regulation of apoptosis
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- View More GO Terms
- GO:0016575 histone deacetylation
- GO:0042826 histone deacetylase binding
- GO:0042921 glucocorticoid receptor signaling pathway
- GO:0050847 progesterone receptor signaling pathway
- GO:0060766 negative regulation of androgen receptor signaling pathway
View more products with Prohibitin specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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