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Prohibitin antibody

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Western Blot Validated
Anti Prohibitin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with prohibitin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to prohibitin (molecular weight 32 kDa)

Mouse anti Prohibitin

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00284
Datasheet
WB
SDS
100 µl loader

Product Details

Target Species
Human
Western Blotting
Anti prohibitin detects a band of approximately 32 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P35232
Entrez Gene
PHB
GO Terms
GO:0006260 DNA replication
GO:0005887 integral to plasma membrane
GO:0005654 nucleoplasm
GO:0005743 mitochondrial inner membrane
GO:0008285 negative regulation of cell proliferation
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0042981 regulation of apoptosis
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0016575 histone deacetylation
GO:0042826 histone deacetylase binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0050847 progesterone receptor signaling pathway
GO:0060766 negative regulation of androgen receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Prohibitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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