Prohibitin antibody

100% Secure

Mouse anti Prohibitin

Prohibitin antibody recognizes Prohibitin, also known as PHB, epididymis luminal protein 215 or epididymis secretory sperm binding protein Li 54e. This antibody has been extensively validated for western blotting using whole cell lysates.

Prohibitin Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00284	WB	100 µl
VMA00284KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human prohibitin antibody recognizes prohibitin, also known as epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e.

The PHB gene is evolutionarily conserved. Encoded by this gene, prohibitin is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of PHB have been identified. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013).

Mouse anti Human prohibitin antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti prohibitin detects a band of approximately 32 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites

Preparation

20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Purified recombinant fragment of human prohibitin expressed in E. coli

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P35232 Related reagents
Entrez Gene: PHB Related reagents
GO Terms: GO:0006260 DNA replication; GO:0005887 integral to plasma membrane; GO:0005654 nucleoplasm; GO:0005743 mitochondrial inner membrane; GO:0008285 negative regulation of cell proliferation; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0042981 regulation of apoptosis; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0016575 histone deacetylation; GO:0042826 histone deacetylase binding; GO:0042921 glucocorticoid receptor signaling pathway; GO:0050847 progesterone receptor signaling pathway; GO:0060766 negative regulation of androgen receptor signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Prohibitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information

Online Payment Methods:

Invoice / Purchase Order
Credit Card (Maestro, VISA,MasterCard & VISA Electron)

View more products with Prohibitin specificity