Prohibitin antibody

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Mouse anti Prohibitin

Prohibitin antibody recognizes Prohibitin, also known as PHB, epididymis luminal protein 215 or epididymis secretory sperm binding protein Li 54e. This antibody has been extensively validated for western blotting using whole cell lysates.

Prohibitin Antibody with Control Lysate

Prohibitin antibody recognizes Prohibitin, also known as PHB, epididymis luminal protein 215 or epididymis secretory sperm binding protein Li 54e. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00284 WB 100 µl
VMA00284KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human prohibitin antibody recognizes prohibitin, also known as epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e.

The PHB gene is evolutionarily conserved. Encoded by this gene, prohibitin is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of PHB have been identified. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013).

Mouse anti Human prohibitin antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti prohibitin detects a band of approximately 32 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human prohibitin expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35232 Related reagents
Entrez Gene
PHB Related reagents
GO Terms
GO:0006260 DNA replication
GO:0005887 integral to plasma membrane
GO:0005654 nucleoplasm
GO:0005743 mitochondrial inner membrane
GO:0008285 negative regulation of cell proliferation
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0042981 regulation of apoptosis
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0016575 histone deacetylation
GO:0042826 histone deacetylase binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0050847 progesterone receptor signaling pathway
GO:0060766 negative regulation of androgen receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Prohibitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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