Progesterone Receptor antibody | 1A6

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Mouse anti Human Progesterone Receptor

Product Type
Monoclonal Antibody
Clone
1A6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1800 P* WB 1 ml
Mouse anti Human Progesterone Receptor antibody, clone 1A6 recognizes the human progesterone receptor which is a member of the steroid family of nuclear receptors. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy.

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
15mMSodium Azide
Immunogen
Synthetic peptide corresponding to a site of predicted high antigenicity on the human progesterone receptor.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P06401 Related reagents
Entrez Gene
PGR Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005102 receptor binding
GO:0005496 steroid binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0007267 cell-cell signaling
GO:0008270 zinc ion binding
GO:0019899 enzyme binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
Regulatory
For research purposes only

Applications of Progesterone Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/40
Western Blotting 1/25 1/50
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Uterus

Product Specific References

References for Progesterone Receptor antibody

  1. Ambroise, M. et al. (2011) Immunohistochemical profile of breast cancer patients at a tertiary care hospital in South India.
    Asian Pac J Cancer Prev. 12: 625-9.