Pro Desmoglein 2 antibody | 20G1
Mouse anti Human pro desmoglein 2 recognizes pro desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.
Desmoglein 2 is a membrane glycoprotein with 4 highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human pro desmoglein 2 specifically recognizes an epitope within the proregion domain of desmoglein 2, epitope mapping having demonstrated reactivity with fusion proteins containing the entire prodomain but not with fusion proteins lacking the proregion (Keim et al., 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
Desmoglein 2 is a membrane glycoprotein with 4 highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human pro desmoglein 2 specifically recognizes an epitope within the proregion domain of desmoglein 2, epitope mapping having demonstrated reactivity with fusion proteins containing the entire prodomain but not with fusion proteins lacking the proregion (Keim et al., 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
Product Details
- Target Species
- Human
- Western Blotting
- Anti pro desmoglein 2 antibody detects a band of approximately 150 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14126
- Entrez Gene
- DSG2
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007156 homophilic cell adhesion
- GO:0030057 desmosome
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Pro Desmoglein 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Product Specific References
References for Pro Desmoglein 2 antibody
-
Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
Hybridoma 27: 249-58.
