Pro Desmoglein 2 antibody | 20G1
Mouse anti Pro Desmoglein 2
Pro-desmoglein 2 antibody recognizes desmoglein-2, also known as HDGC or cadherin family member 5. This antibody has been extensively validated for western blotting using whole cell lysates.
Pro Desmoglein 2 Antibody with Control Lysate
Pro-desmoglein 2 antibody recognizes desmoglein-2, also known as HDGC or cadherin family member 5. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- 20G1
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00094 | WB | 100 µl | ||||
| VMA00094KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human pro desmoglein 2 recognizes pro desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.
Desmoglein 2 is a membrane glycoprotein with 4 highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human pro desmoglein 2 specifically recognizes an epitope within the proregion domain of desmoglein 2, epitope mapping having demonstrated reactivity with fusion proteins containing the entire prodomain but not with fusion proteins lacking the proregion (Keim et al., 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
Desmoglein 2 is a membrane glycoprotein with 4 highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human pro desmoglein 2 specifically recognizes an epitope within the proregion domain of desmoglein 2, epitope mapping having demonstrated reactivity with fusion proteins containing the entire prodomain but not with fusion proteins lacking the proregion (Keim et al., 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
Product Details
- Target Species
- Human
- Western Blotting
- Anti pro desmoglein 2 antibody detects a band of approximately 150 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14126 Related reagents
- Entrez Gene
- DSG2 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007156 homophilic cell adhesion
- GO:0030057 desmosome
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Pro Desmoglein 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Western Blotting | 1/1000 | ||
| Immunofluorescence | |||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Product Specific References
References for Pro Desmoglein 2 antibody
-
Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
Hybridoma 27: 249-58.
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