Pro Desmoglein 2 antibody | 19B9

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Mouse anti Pro Desmoglein 2

Product Type
PrecisionAb Monoclonal
Clone
19B9
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00093
Datasheet
IF WB
MSDS
100 µl loader

Mouse anti-Human pro desmoglein 2 recognises pro desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.

Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.

Mouse anti Human pro desmoglein 2 antibody has been found to be specific for a region near the N terminus of EC1 but only binds to the target in a proregion-dependent manner (Keim et al. 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.

Product Details

Target Species
Human
Western Blotting
Anti pro desmoglein 2 antibody detects a band of approximately 150 kDa in Western Blotting
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14126 Related reagents
Entrez Gene
DSG2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Pro Desmoglein 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Product Specific References

References for Pro Desmoglein 2 antibody

  1. Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
    Hybridoma (Larchmt). 27 (4): 249-58.

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