Pro Desmoglein 2 antibody | 19B9

100% Secure


Mouse anti Pro Desmoglein 2

Mouse anti-human desmoglein-2 antibody recognizes desmoglein-2, also known as HDGC or cadherin family member 5. This antibody has been extensively validated for western blotting using whole cell lysates.

Pro Desmoglein 2 Antibody with Control Lysate

Mouse anti-human desmoglein-2 antibody recognizes desmoglein-2, also known as HDGC or cadherin family member 5. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
19B9
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00093 IF WB 100 µl
VMA00093KT WB 2 Westerns
With Lysate 400µg
Mouse anti-Human pro desmoglein 2 recognises pro desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.

Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.

Mouse anti Human pro desmoglein 2 antibody has been found to be specific for a region near the N terminus of EC1 but only binds to the target in a proregion-dependent manner (Keim et al. 2008). This antibody has been extensively validated for western blotting using whole cell lysates and is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.

Product Details

Target Species
Human
Western Blotting
Anti pro desmoglein 2 antibody detects a band of approximately 150 kDa in Western Blotting
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A431 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14126 Related reagents
Entrez Gene
DSG2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Pro Desmoglein 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Product Specific References

References for Pro Desmoglein 2 antibody

  1. Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
    Hybridoma (Larchmt). 27 (4): 249-58.

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted