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PRKAR2A antibody | OTI4A3

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Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRKAR2A antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to PRKAR2A (molecular weight 51 kDa)
Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of PRKAR2A knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti PRKAR2A antibody (VMA00718) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 375 second and (B) 30 second exposure.
Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of PRKAR2A from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti PRKAR2A

Product Type
PrecisionAb Monoclonal
Clone
OTI4A3
Isotype
IgG1
Format
Purified
Specificity
PRKAR2A

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Product Code Applications Pack Size List Price Your Price Qty
VMA00718
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human PRKAR2A antibody recognizes PRKAR2A, also known as PKR2, cAMP-dependent protein kinase regulatory subunit RII alpha and protein kinase A RII-alpha subunit.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by PRKAR2A is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) (provided by RefSeq, Jul 2008).

Mouse anti Human PRKAR2A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti PRKAR2A detects a band of approximately 51 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
HEK293-derived full length recombinant protein of human PRKAR2A
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P13861
Entrez Gene
PRKAR2A
GO Terms
GO:0001932 regulation of protein phosphorylation
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0005624 membrane fraction
GO:0006112 energy reserve metabolic process
GO:0006833 water transport
GO:0007202 activation of phospholipase C activity
GO:0050796 regulation of insulin secretion
GO:0008603 cAMP-dependent protein kinase regulator activity
GO:0030552 cAMP binding
GO:0034199 activation of protein kinase A activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0055085 transmembrane transport
GO:0071377 cellular response to glucagon stimulus

VMA00718

170727

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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