PRKAB1 antibody | K02/3D4

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Mouse anti PRKAB1

Product Type
PrecisionAb Monoclonal
Clone
K02/3D4
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00924
Datasheet
WB
SDS
100 µl loader

Mouse anti PRKAB1 antibody recognizes 5'-AMP-activated protein kinase subunit beta-1, also known as AMPKb.

AMPK is a serine/threonine protein kinase complex which acts as a sensor of adenine nucleotides, and is activated by low cellular energy. Under these conditions AMPK activates catabolic pathways that generate ATP and deactivates anabolic pathways that consume ATP, restoring energy balance (Lin et al. 2017). Conditions that deplete cellular energy levels include nutrient starvation, hypoxia, and exposure to toxins, inhibiting the mitochondrial respiratory chain and activating AMPK. Activators and inhibitors of AMPK have been suggested as important tools for controlling human diseases including metabolic syndrome and cancer (Kim et al. 2016). In mice, AMPK appears to act as a tumor suppressor and a tumor promoter in different contexts. Prior to cancer development it can act as a suppressor, but switches to being a tumor promoter once cancer has occurred, favoring survival of cancer cells (Vara-Ciruelos et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti PRKAB1 detects a band of approximately 36 kDa in C6 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-270 of human PRKAB1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y478
Entrez Gene
PRKAB1
GO Terms
GO:0005829 cytosol
GO:0006633 fatty acid biosynthetic process
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0046320 regulation of fatty acid oxidation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PRKAB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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