PRKAB1 antibody | K02/3D4
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WB ResetMouse anti PRKAB1
- Product Type
- PrecisionAb Monoclonal
- Clone
- K02/3D4
- Isotype
- IgG1
- Format
- Purified
- Specificity
- PRKAB1
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| Mouse anti PRKAB1 antibody recognizes 5'-AMP-activated protein kinase subunit beta-1, also known as AMPKb. AMPK is a serine/threonine protein kinase complex which acts as a sensor of adenine nucleotides, and is activated by low cellular energy. Under these conditions AMPK activates catabolic pathways that generate ATP and deactivates anabolic pathways that consume ATP, restoring energy balance (Lin et al. 2017). Conditions that deplete cellular energy levels include nutrient starvation, hypoxia, and exposure to toxins, inhibiting the mitochondrial respiratory chain and activating AMPK. Activators and inhibitors of AMPK have been suggested as important tools for controlling human diseases including metabolic syndrome and cancer (Kim et al. 2016). In mice, AMPK appears to act as a tumor suppressor and a tumor promoter in different contexts. Prior to cancer development it can act as a suppressor, but switches to being a tumor promoter once cancer has occurred, favoring survival of cancer cells (Vara-Ciruelos et al. 2019). |
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- Target Species
- Human
- Western Blotting
- Mouse anti PRKAB1 detects a band of approximately 36 kDa in C6 cell lysates
- Species Cross-Reactivity
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Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-270 of human PRKAB1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q9Y478
- Entrez Gene
- PRKAB1
- GO Terms
- GO:0005829 cytosol
- GO:0006633 fatty acid biosynthetic process
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0046320 regulation of fatty acid oxidation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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