PRAS40 antibody
Mouse anti Human PRAS40 antibody recognizes PRAS40, also known as 40 kDa proline-rich AKT substrate.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).
Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).
Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified His-tagged fragment of the PRAS40 protein
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q96B36
- Entrez Gene
- AKT1S1
- GO Terms
- GO:0005515 protein binding
- GO:0006469 negative regulation of protein kinase activity
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032007 negative regulation of TOR signaling cascade
- GO:0043526 neuroprotection
- GO:0044445 cytosolic part
- GO:0045792 negative regulation of cell size
- GO:0045884 regulation of survival gene product expression
- GO:0048011 nerve growth factor receptor signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PRAS40 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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