PRAS40 antibody

100% Secure

Mouse anti PRAS40

Mouse anti-human PRAS40 antibody recognizes proline-rich Akt1 substrate 1, also known as 40 kDa proline-rich Akt substrate. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00493 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human PRAS40 antibody recognizes PRAS40, also known as 40 kDa proline-rich AKT substrate.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).

Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified His-tagged fragment of the PRAS40 protein
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q96B36 Related reagents
Entrez Gene: AKT1S1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0006469 negative regulation of protein kinase activity; GO:0048015 phosphatidylinositol-mediated signaling; GO:0032007 negative regulation of TOR signaling cascade; GO:0043526 neuroprotection; GO:0044445 cytosolic part; GO:0045792 negative regulation of cell size; GO:0045884 regulation of survival gene product expression; GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PRAS40 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.