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PRAS40 antibody

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Western Blot Validated
Anti PRAS40 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRAS40 antibody (VMA00493) followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 18 second exposure. Arrow points to PRAS40 (molecular weight 40 kDa).

Mouse anti PRAS40

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00493
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human PRAS40 antibody recognizes PRAS40, also known as 40 kDa proline-rich AKT substrate.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).

Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified His-tagged fragment of the PRAS40 protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q96B36
Entrez Gene
AKT1S1
GO Terms
GO:0005515 protein binding
GO:0006469 negative regulation of protein kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032007 negative regulation of TOR signaling cascade
GO:0043526 neuroprotection
GO:0044445 cytosolic part
GO:0045792 negative regulation of cell size
GO:0045884 regulation of survival gene product expression
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PRAS40 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the PRAS40 specificity