AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).
Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Purified His-tagged fragment of the PRAS40 protein
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- Q96B36 Related reagents
- Entrez Gene
- AKT1S1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0006469 negative regulation of protein kinase activity
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032007 negative regulation of TOR signaling cascade
- GO:0043526 neuroprotection
- GO:0044445 cytosolic part
- GO:0045792 negative regulation of cell size
- GO:0045884 regulation of survival gene product expression
- GO:0048011 nerve growth factor receptor signaling pathway
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of PRAS40 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
Western Blotting without Trial and Error: Use PrecisionAb Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.