PRAS40 antibody
Mouse anti PRAS40
Mouse anti-human PRAS40 antibody recognizes proline-rich Akt1 substrate 1, also known as 40 kDa proline-rich Akt substrate. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00493 | WB | 100 µl |
Mouse anti Human PRAS40 antibody recognizes PRAS40, also known as 40 kDa proline-rich AKT substrate.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).
Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).
Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified His-tagged fragment of the PRAS40 protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q96B36 Related reagents
- Entrez Gene
- AKT1S1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0006469 negative regulation of protein kinase activity
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032007 negative regulation of TOR signaling cascade
- GO:0043526 neuroprotection
- GO:0044445 cytosolic part
- GO:0045792 negative regulation of cell size
- GO:0045884 regulation of survival gene product expression
- GO:0048011 nerve growth factor receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PRAS40 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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