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PRAS40 antibody

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Anti PRAS40 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti PRAS40 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRAS40 antibody (VMA00493) followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 18 second exposure. Arrow points to PRAS40 (molecular weight 40 kDa).

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WB

Mouse anti PRAS40

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
PRAS40

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00493
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader
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Mouse anti Human PRAS40 antibody recognizes PRAS40, also known as 40 kDa proline-rich AKT substrate.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. 2003).

Mouse anti Human PRAS40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PRAS40 detects a band of approximately 40 kDa in HEK293 cell lysate.
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified His-tagged fragment of the PRAS40 protein
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q96B36
Entrez Gene
AKT1S1
GO Terms
GO:0005515 protein binding
GO:0006469 negative regulation of protein kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032007 negative regulation of TOR signaling cascade
GO:0043526 neuroprotection
GO:0044445 cytosolic part
GO:0045792 negative regulation of cell size
GO:0045884 regulation of survival gene product expression
GO:0048011 nerve growth factor receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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