PPP2CB antibody | AB02/4B4

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Mouse anti PPP2CB

Product Type
PrecisionAb Monoclonal
Clone
AB02/4B4
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00814
Datasheet
WB
SDS
100 µl loader

Mouse anti PPP2CB antibody recognizes serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, also known as PP2A-beta.

PPP2CB is a major serine/threonine phosphatase with regulatory roles in numerous cellular pathways (Eydmann and Knowles 1997). During mammalian oocyte development, PPP2CB upregulation is involved with the meiotic arrest phenotype and female infertility (Su et al. 2012). PPP2CB has been identified as a potential gene involved with pathogenesis of Parkinson's disease when mutated (Guo et al. 2018). PPP2CB is also a tumor suppressor, and targeting of PPP2CB via miRNA-1246 has been found to initiate pro-inflammatory responses in mesenchymal stem/stromal cells (Bott et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti PPP2CB antibody detects a band of approximately 34 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-309 of human PPP2CB
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P62714
Entrez Gene
PPP2CB
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0000775 chromosome, centromeric region
GO:0000922 spindle pole
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006470 protein dephosphorylation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPP2CB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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