PPP2CA antibody | AB02/4H8

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Mouse anti PPP2CA

PPP2CA antibody recognizes PPP2CA, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C.

PPP2CA Antibody with Control Lysate

PPP2CA antibody recognizes PPP2CA, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C.

Product Type
PrecisionAb™ Monoclonal
Clone
AB02/4H8
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00680 WB 100 µl
VMA00680KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PPP2CA antibody recognizes PPP2CA, also known as serine/threonine-protein phosphatase 2A catalytic subunit, PP2A-alpha, protein phosphatase 2A catalytic and replication protein C.

PPP2CA encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).

Mouse anti Human PPP2CA detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-309 of human PPP2CA
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P67775 Related reagents
Entrez Gene
PPP2CA Related reagents
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0000188 inactivation of MAPK activity
GO:0000775 chromosome, centromeric region
GO:0000922 spindle pole
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004721 phosphoprotein phosphatase activity
GO:0046872 metal ion binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006275 regulation of DNA replication
GO:0006470 protein dephosphorylation
GO:0006672 ceramide metabolic process
GO:0006917 induction of apoptosis
GO:0007126 meiosis
GO:0008380 RNA splicing
GO:0010033 response to organic substance
GO:0010719 negative regulation of epithelial to mesenchymal transition
GO:0019932 second-messenger-mediated signaling
GO:0030111 regulation of Wnt receptor signaling pathway
GO:0030155 regulation of cell adhesion
GO:0030308 negative regulation of cell growth
GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein
GO:0045449 regulation of transcription
GO:0071902 positive regulation of protein serine/threonine kinase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPP2CA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti PPP2CA detects a band of approximately 32 kDa in Jurkat cell lysates
Western Blotting
Mouse anti PPP2CA detects a band of approximately 32 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB