PPM1G antibody | OTI1C12

100% Secure


Mouse anti PPM1G

Mouse anti human PPM1G antibody recognizes phosphatase 1G, also known as Mg2+/Mn2+ dependent 1G, PP2C gamma, PP2CG, protein phosphatase 2, catalytic subunit, gamma isoform or protein phosphatase 2C isoform gamma. This antibody has been validated for western blotting using whole cell lysates.

PPM1G Antibody with Control Lysate

Mouse anti human PPM1G antibody recognizes phosphatase 1G, also known as Mg2+/Mn2+ dependent 1G, PP2C gamma, PP2CG, protein phosphatase 2, catalytic subunit, gamma isoform or protein phosphatase 2C isoform gamma. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1C12
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00427 WB 100 µl
VMA00427KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PPM1G antibody recognizes the PPM1G, also known as protein phosphatase Mg2+/Mn2+ dependent 1G, PP2C gamma, PP2CG, protein phosphatase 1G (formerly 2C) and protein phosphatase 2C isoform gamma.

The protein encoded by the PPM1G gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression (provided by RefSeq, Apr 2010).

Mouse anti Human PPM1G antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PPM1G detects a band of approximately 59 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human PPM1G (NP_817092) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg NIH/3T3 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O15355 Related reagents
Entrez Gene
PPM1G Related reagents
GO Terms
GO:0004722 protein serine/threonine phosphatase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006470 protein dephosphorylation
GO:0007050 cell cycle arrest
GO:0008287 protein serine/threonine phosphatase complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPM1G antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted