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PPM1A antibody | 7F12

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Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PPM1A antibody (VMA00163) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 33 second exposure. Arrow points to PPM1A (molecular weight 42 kDa).
Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of PPM1A knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti PPM1A antibody (VMA00163) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 112 second and (B) 30 second exposure.
Anti PPM1A Antibody, clone 7F12 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of PPM1A from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti PPM1A

Product Type
PrecisionAb Monoclonal
Clone
7F12
Isotype
IgG1
Format
Purified
Specificity
PPM1A

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Product Code Applications Pack Size List Price Your Price Qty
VMA00163
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human PPM1A antibody recognizes protein phosphatase 1A, also known as protein phosphatase 1A magnesium-dependent, alpha isoform.

Protein phosphatase 1A, encoded by the PPM1A gene, is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described (provided by RefSeq, Jul 2008).

Mouse anti Human PPM1A antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PPM1A detects a band of approximately 42 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human PPM1A, amino acids 202-382, expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P35813
Entrez Gene
PPM1A
GO Terms
GO:0000287 magnesium ion binding
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0004871 signal transducer activity
GO:0016055 Wnt receptor signaling pathway
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0008287 protein serine/threonine phosphatase complex
GO:0030145 manganese ion binding
GO:0030177 positive regulation of Wnt receptor signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045893 positive regulation of transcription, DNA-dependent

VMA00163

120615

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