Poly (ADP-Ribose) Polymerase 1 antibody

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Mouse anti Poly (ADP-Ribose) Polymerase 1

Poly (ADP-ribose) polymerase antibody recognizes Poly (ADP-ribose) polymerase 1, also known as PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1 or Poly(ADP-ribose) synthase 1. The antibody has been extensively validated for western blotting.

Poly (ADP-Ribose) Polymerase 1 Antibody with Control Lysate

Poly (ADP-ribose) polymerase antibody recognizes Poly [ADP-ribose] polymerase 1, also known as PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1 or Poly[ADP-ribose] synthase 1.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00016 WB 100 µl
VMA00016KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme cleaved during apoptosis (Soldani et al. 2002).

PARP-1 is a caretaker enzyme involved in DNA damage repair (Langelier et al. 2013). It plays roles in diabetes pathophysiology (Andreone et al. 2012) and tumor proliferation (Rosado et al 2013). As well as protecting cells from genomic instability, PARP-1 is also involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis (Erdélyi et al. 2005).

PARP-1 represents a promising target for new developments in therapeutic treatment of immune mediated diseases (Rosado et al. 2013) and has considerable potential for delivering selective tumor cell killing while sparing normal cells (Pinton et al. 2013).

Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes PARP-1 as a single band of ~116 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.

Product Details

Target Species
Human
Western Blotting
Anti Poly (ADP-Ribose) Polymerase 1 antibody detects a band of approximately 116 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human PARP-1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P09874 Related reagents
Entrez Gene
PARP1 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005635 nuclear envelope
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0006366 transcription from RNA polymerase II promoter
GO:0006471 protein ADP-ribosylation
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0032869 cellular response to insulin stimulus
GO:0045449 regulation of transcription
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Poly (ADP-Ribose) Polymerase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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