Poly ADP-Ribose antibody | 10H
Mouse anti Human poly ADP ribose antibody, clone 10H (MCA1480) used to illustrate PAR expression in rabbit endocardial tissue section by immunohistochemistry on formalin fixed paraffin embedded tissue sections following antigen retrieval in citrate buffer.
Image caption:
(A) Staining and quantification of nuclear PAR (poly-ADP-ribose) expression. Bar graphs depict percentage of nuclei positively stained for PAR in specimens from left ventricular epicardium, myocardium, and endocardium and right ventricle after 90 min of cardioplegia followed by 60 min of recovery. All data are given as means ± SEM. Significant differences (p<0.05) between CP− and CP+ are indicated by asterisks (*). (B) Original PAR-staining of left ventricular (LV) endocardium. Arrows indicate cell nuclei positive for PAR (stained in red).
From: Salameh A, Keller M, Dähnert I, Dhein S.
Olesoxime Inhibits Cardioplegia-Induced Ischemia/Reperfusion Injury. A Study in Langendorff-Perfused Rabbit Hearts.
Front Physiol. 2017 May 19;8:324.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Poly(Adp-Ribose) antibody, clone 10H recognizes poly (ADP-ribose) modified proteins. The antibody does not cross-react with RNA, DNA or monomers of ADP-ribose.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 50% Glycerol
- Immunogen
- Purified poly (ADP-ribose) polymer, 10-50 unit chain length
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Poly ADP-Ribose antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | 1/500 |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Poly ADP-Ribose antibody
-
Kawamitsu, H. et al. (1984) Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.
Biochemistry. 23 (16): 3771-7. -
Love, S. et al. (1999) Increased poly(ADP-ribosyl)ation of nuclear proteins in Alzheimer's disease.
Brain. 122 ( Pt 2): 247-53. -
Salameh, A. et al. (2017) Olesoxime Inhibits Cardioplegia-Induced Ischemia/Reperfusion Injury. A Study in Langendorff-Perfused Rabbit Hearts.
Front Physiol. 8: 324.
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