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Poly ADP-Ribose antibody | 10H

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Anti Human Poly(ADP-Ribose) Antibody, clone 10H gallery image 1
Published customer image:
Mouse anti Human poly ADP ribose antibody, clone 10H (MCA1480) used to illustrate PAR expression in rabbit endocardial tissue section by immunohistochemistry on formalin fixed paraffin embedded tissue sections following antigen retrieval in citrate buffer.
Image caption:
(A) Staining and quantification of nuclear PAR (poly-ADP-ribose) expression. Bar graphs depict percentage of nuclei positively stained for PAR in specimens from left ventricular epicardium, myocardium, and endocardium and right ventricle after 90 min of cardioplegia followed by 60 min of recovery. All data are given as means ± SEM. Significant differences (p<0.05) between CP− and CP+ are indicated by asterisks (*). (B) Original PAR-staining of left ventricular (LV) endocardium. Arrows indicate cell nuclei positive for PAR (stained in red).

From: Salameh A, Keller M, Dähnert I, Dhein S.
Olesoxime Inhibits Cardioplegia-Induced Ischemia/Reperfusion Injury. A Study in Langendorff-Perfused Rabbit Hearts.
Front Physiol. 2017 May 19;8:324.

Mouse anti Human Poly(ADP-Ribose)

Product Type
Monoclonal Antibody
Clone
10H
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1480 IP P* WB 0.1 ml
Mouse anti Human Poly(Adp-Ribose) antibody, clone 10H recognizes poly (ADP-ribose) modified proteins. The antibody does not cross-react with RNA, DNA or monomers of ADP-ribose.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
Immunogen
Purified poly (ADP-ribose) polymer, 10-50 unit chain length
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Poly ADP-Ribose antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 1/500
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG3:FITC HCA039F 0.1 mg F
Human anti Mouse IgG3:HRP HCA039P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Poly ADP-Ribose antibody

  1. Kawamitsu, H. et al. (1984) Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.
    Biochemistry. 23 (16): 3771-7.
  2. Love, S. et al. (1999) Increased poly(ADP-ribosyl)ation of nuclear proteins in Alzheimer's disease.
    Brain. 122 ( Pt 2): 247-53.
  3. Salameh, A. et al. (2017) Olesoxime Inhibits Cardioplegia-Induced Ischemia/Reperfusion Injury. A Study in Langendorff-Perfused Rabbit Hearts.
    Front Physiol. 8: 324.
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