POLR2E antibody | CD01/1F2

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Mouse anti POLR2E

Product Type
PrecisionAb Monoclonal
Clone
CD01/1F2
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00880
Datasheet
WB
SDS
100 µl loader

Mouse anti POLR2E antibody recognizes DNA-directed RNA polymerases I, II, and III subunit RPABC1, also known as polymerase II polypeptide E.

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. The rs3787016 polymorphism of POLR2E has been suggested as a potential biomarker for cancer disposition (Chen et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti POLR2E antibody detects a band of approximately 23 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-210 of human POLR2E
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P19388
Entrez Gene
POLR2E
GO Terms
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003899 DNA-directed RNA polymerase activity
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0006385 transcription elongation from RNA polymerase III promoter
GO:0006386 termination of RNA polymerase III transcription
GO:0016032 viral reproduction
GO:0044419 interspecies interaction between organisms
GO:0050434 positive regulation of viral transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of POLR2E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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