POLR2E antibody | CD01/1F2
Mouse anti POLR2E antibody recognizes DNA-directed RNA polymerases I, II, and III subunit RPABC1, also known as polymerase II polypeptide E.
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. The rs3787016 polymorphism of POLR2E has been suggested as a potential biomarker for cancer disposition (Chen et al. 2018).
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. The rs3787016 polymorphism of POLR2E has been suggested as a potential biomarker for cancer disposition (Chen et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti POLR2E antibody detects a band of approximately 23 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-210 of human POLR2E
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19388
- Entrez Gene
- POLR2E
- GO Terms
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0005665 DNA-directed RNA polymerase II, core complex
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006370 mRNA capping
- GO:0006385 transcription elongation from RNA polymerase III promoter
- GO:0006386 termination of RNA polymerase III transcription
- GO:0016032 viral reproduction
- GO:0044419 interspecies interaction between organisms
- GO:0050434 positive regulation of viral transcription
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of POLR2E antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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