POLR2A antibody | AB01/3D9

100% Secure


Mouse anti POLR2A

POLR2A antibody recognizes RNA Polymerase IIA, also known as DNA-directed RNA polymerase II subunit A, RPB1 or RPO2.

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/3D9
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00613
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human POLR2A antibody recognizes RNA polymerase IIA, also known as DNA-directed RNA polymerase II subunit A, RPB1 or RPO2.

The POLR2A gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA (provided by RefSeq, Jul 2008).

Mouse anti Human POLR2A antibody detects a band of 250 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti POLR2A antibody detects a band of approximately 250 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide containing 6 repeats of the amino acid sequence YSPTSPS
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P24928 Related reagents
Entrez Gene
POLR2A Related reagents
GO Terms
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003899 DNA-directed RNA polymerase activity
GO:0003968 RNA-directed RNA polymerase activity
GO:0046872 metal ion binding
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006355 regulation of transcription, DNA-dependent
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0016032 viral reproduction
GO:0031625 ubiquitin protein ligase binding
GO:0050434 positive regulation of viral transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of POLR2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.