Podoplanin antibody | D2-40

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Mouse anti Human Podoplanin

Product Type
Monoclonal Antibody
Clone
D2-40
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2543 C IF P WB 1 ml
Mouse anti human podoplanin antibody, clone D2-40 was raised against M2A antigen (Marks et al, 1999) and detects podoplanin (Sonne et al, 2006). Podoplanin (PDPN) is an O-glycosylated transmembrane glycoprotein that is selectively expressed by, and is a marker of, lymphatic endothelial cells. In normal tissue the ~38 kDa protein is also present in human lung, placenta, heart, skeletal muscle and kidney podocytes. It is not found in the blood vasculature (Breiteneder-Geleff et al, 1999, Wicki and Christofori, 2007).

The function of podoplanin is yet to be fully elucidated; however, it may be involved in cell migration and/or actin cytoskeleton organization. It is required for normal lung cell proliferation and alveolus formation at birth, and can induce platelet aggregation (Ramirez et al, 2003, Wicki and Christofori, 2007).

Mouse anti human podoplanin antibody, clone D2-40 has been shown to be a sensitive and specific antibody for the detection of lymphatic endothelium in different malignancies, and is of value in the routine evaluation of lymphatic invasion in esophageal cancer (Kozłowski et al, 2011). Clone D2-40 was reported to be an excellent immunohistochemical marker of cutaneous Kaposi’s sarcomas, (Kahn et al, 2002), and may be useful in the differential diagnosis of epithelioid malignant mesothelioma versus adenocarcinoma (Chu et al, 2005).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Resected tissue from dysgeminoma of the ovary

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
Q86YL7 Related reagents
Entrez Gene
PDPN Related reagents
GO Terms
GO:0000902 cell morphogenesis
GO:0001946 lymphangiogenesis
GO:0005887 integral to plasma membrane
GO:0008360 regulation of cell shape
GO:0031258 lamellipodium membrane
GO:0031527 filopodium membrane
GO:0031528 microvillus membrane
GO:0032587 ruffle membrane
Regulatory
For research purposes only

Applications of Podoplanin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/10 1/40
Immunohistology - Paraffin 1/10 1/40
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human lymph node

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for Podoplanin antibody

  1. Marks, A. et al. (1999) Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours.
    Br J Cancer. 80 (3-4): 569-78.
  2. Wu, H.M. et al. (2013) Expression of podoplanin in salivary gland adenoid cystic carcinoma and its association with distant metastasis and clinical outcomes.
    Mol Med Rep. 6: 271-4.
  3. Takagi, S. et al. (2013) Platelets promote tumor growth and metastasis via direct interaction between Aggrus/podoplanin and CLEC-2.
    PLoS One. 8: e73609.
  4. Sonne, S.B. et al. (2006) Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours.
    Virchows Arch. 449 (2): 200-6.
  5. Heindl, L.M. et al (2010) Intraocular tumor-associated lymphangiogenesis a novel prognostic factor for ciliary body melanomas with extraocular extension.
    Ophthalmology 117(2): 334-42.
  6. Ekwall, A.K. et al. (2011) The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis.
    Arthritis Res Ther. 13:R40.
  7. Kozłowski, M. et al. (2011) Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer.
    Folia Histochem Cytobiol. 49: 90-7.
  8. Rudzińska, M. et al. (2014) The role of podoplanin in the biology of differentiated thyroid cancers.
    PLoS One. 9: e96541.
  9. Bolzoni Villaret, A. et al. (2010) Immunostaining patterns of CD31 and podoplanin in previously untreated advanced oral/oropharyngeal cancer: prognostic implications.
    Head Neck. 32: 786-92.
  10. Heindl, L.M. et al. (2014) Lack of ciliary body lymphatics in iridociliary melanocytoma.
    Graefes Arch Clin Exp Ophthalmol. 252: 169-71.
  11. Heindl, L.M. et al. (2009) Intraocular lymphangiogenesis in malignant melanomas of the ciliary body with extraocular extension.
    Invest Ophthalmol Vis Sci. 50: 1988-95.
  12. Heindl, L.M. et al. (2010) Intraocular tumor-associated lymphangiogenesis a novel prognostic factor for ciliary body melanomas with extraocular extension?
    Ophthalmology. 117: 334-42.
  13. Wessel, J.M. et al. (2012) Invasion of lymphatic vessels into the eye after open globe injuries.
    Invest Ophthalmol Vis Sci. 53: 3717-25.
  14. Heindl, L.M. et al. (2011) Tumor-associated lymphangiogenesis in the development of conjunctival melanoma.
    Invest Ophthalmol Vis Sci. 52: 7074-83.
  15. Bolzoni Villaret, A. et al. (2013) Angiogenesis and lymphangiogenesis in early-stage laryngeal carcinoma: Prognostic implications.
    Head Neck. 35: 1132-7.
  16. Heindl, L.M. et al. (2011) Prognostic significance of tumor-associated lymphangiogenesis in malignant melanomas of the conjunctiva.
    Ophthalmology. 118: 2351-60.
  17. Miyata, K. et al. (2014) Suppression of Aggrus/podoplanin-induced platelet aggregation and pulmonary metastasis by a single-chain antibody variable region fragment.
    Cancer Med. 3 (6): 1595-604.
  18. Choi, I.Y. et al. (2017) Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis.
    PLoS One. 12 (8): e0182751.