Podoplanin antibody | AB01/1B6
Mouse anti Podoplanin antibody recognizes podoplanin, also known as aggrus, glycoprotein 36, and T1-alpha.
Podoplanin is a mucin-type transmembrane receptor required for correct formation of the lungs and lymphatic system during embryogenesis. Although discovered in the kidney, Podoplanin is expressed in numerous other human tissues including skeletal muscle, heart, placenta, and lung (Sikorska et al. 2019). Podoplanin regulates the activity of proteins including Rho and ezrin, and affects tumor cell motility, invasion and metastasis. Expression of Podoplanin is induced by tumor promoters, and Podoplanin has been suggested as a cancer biomarker and therapeutic agent (Krishnan et al. 2013). Specifically, inhibitors of Podoplanin or the Podoplanin-mediated signaling pathway have been suggested as treatments for small-cell lung cancer (Takahashi et al. 2015).
Podoplanin is a mucin-type transmembrane receptor required for correct formation of the lungs and lymphatic system during embryogenesis. Although discovered in the kidney, Podoplanin is expressed in numerous other human tissues including skeletal muscle, heart, placenta, and lung (Sikorska et al. 2019). Podoplanin regulates the activity of proteins including Rho and ezrin, and affects tumor cell motility, invasion and metastasis. Expression of Podoplanin is induced by tumor promoters, and Podoplanin has been suggested as a cancer biomarker and therapeutic agent (Krishnan et al. 2013). Specifically, inhibitors of Podoplanin or the Podoplanin-mediated signaling pathway have been suggested as treatments for small-cell lung cancer (Takahashi et al. 2015).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti podoplanin antibody detects a band of approximately 39 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-162 of human podoplanin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q86YL7
- Entrez Gene
- PDPN
- GO Terms
- GO:0000902 cell morphogenesis
- GO:0001946 lymphangiogenesis
- GO:0005887 integral to plasma membrane
- GO:0008360 regulation of cell shape
- GO:0031258 lamellipodium membrane
- GO:0031527 filopodium membrane
- GO:0031528 microvillus membrane
- GO:0032587 ruffle membrane
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Podoplanin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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