Plc GAMMA-1 antibody

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Mouse anti Plc GAMMA-1

Plc GAMMA-1 Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00584 WB 100 µl
VMA00584KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PLC gamma-1 antibody recognizes the 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also known as PLC-148, phosphatidylinositol phospholipase C or phosphoinositidase C.

The protein encoded by PLCG1 gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for PLCG1 (provided by RefSeq, Jul 2008).

Mouse anti Human PLC gamma-1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PLC gamma-1 detects a band of approximately 150 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
Buffer Solution
HEPES buffered saline
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human PLC gamma-1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P19174 Related reagents
Entrez Gene
PLCG1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0005829 cytosol
GO:0005057 receptor signaling protein activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007202 activation of phospholipase C activity
GO:0007411 axon guidance
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0009395 phospholipid catabolic process
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0044419 interspecies interaction between organisms
GO:0045766 positive regulation of angiogenesis
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Plc GAMMA-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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