Plc GAMMA-1 antibody
The protein encoded by PLCG1 gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for PLCG1 (provided by RefSeq, Jul 2008).
Mouse anti Human PLC gamma-1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti PLC gamma-1 detects a band of approximately 150 kDa in Jurkat cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- E. coli-derived recombinant human PLC gamma-1
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P19174 Related reagents
- Entrez Gene
- PLCG1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004435 phosphatidylinositol phospholipase C activity
- GO:0005829 cytosol
- GO:0005057 receptor signaling protein activity
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007202 activation of phospholipase C activity
- GO:0007411 axon guidance
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0009395 phospholipid catabolic process
- GO:0043536 positive regulation of blood vessel endothelial cell migration
- GO:0044419 interspecies interaction between organisms
- GO:0045766 positive regulation of angiogenesis
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of Plc GAMMA-1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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