Plakophilin 1 antibody

Mouse anti Plakophilin 1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Plakophilin 1
Region
aa235-726

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VMA00096
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WB 100 µl loader
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Mouse anti Human plakophilin 1 recognizes a member of the plakophilin family which have been identified as junctional proteins that are essential for the formation and stabilization of desmosomal cell contacts. Plakophilin 1 is a protein that in humans is encoded by the PKP1 gene, with a molecular weight of 75 kDa. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families.

Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation and in the formation of desmosomal plaques where it is associated with desmoplakin. It is not found in cell types that have non-epithelial desmosomes and is absent in fibroblasts and other connective tissue types, including sarcomas. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome.

Mouse anti Human plakophilin 1 antibodu has been found to be specific for the armadillo repeat domain of plakophilin 1. This antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant fusion protein fragment, consisting of amino acids 235-726 of human plakophilin 1, fused to maltose binding protein.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

References for Plakophilin 1 antibody

  1. Sobolik-Delmaire et al (2010) Plakophilin-1 Localizes to the Nucleus and Interacts with Single-Stranded DNA-
    Journal of Investigative Dermatology (2010) 130, 2638–2646

UniProt
Q13835
Entrez Gene
PKP1
GO Terms
GO:0005882 intermediate filament
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007275 multicellular organismal development
GO:0019215 intermediate filament binding
GO:0030057 desmosome
GO:0030280 structural constituent of epidermis
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