Plakophilin 1 antibody

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Mouse anti Plakophilin 1

Plakophilin-1 antibody recognizes plakophilin-1, also known as band 6 protein or PKP1. This antibody has been extensively validated for western blotting using whole cell lysates.

Plakophilin 1 Antibody with Control Lysate

Plakophilin-1 antibody recognizes plakophilin-1, also known as band 6 protein or PKP1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00096 WB 100 µl
VMA00096KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human plakophilin 1 recognizes a member of the plakophilin family which have been identified as junctional proteins that are essential for the formation and stabilization of desmosomal cell contacts. Plakophilin 1 is a protein that in humans is encoded by the PKP1 gene, with a molecular weight of 75 kDa. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families.

Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation and in the formation of desmosomal plaques where it is associated with desmoplakin. It is not found in cell types that have non-epithelial desmosomes and is absent in fibroblasts and other connective tissue types, including sarcomas. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome.

Mouse anti Human plakophilin 1 antibodu has been found to be specific for the armadillo repeat domain of plakophilin 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein fragment, consisting of amino acids 235-726 of human plakophilin 1, fused to maltose binding protein.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13835 Related reagents
Entrez Gene
PKP1 Related reagents
GO Terms
GO:0005882 intermediate filament
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007275 multicellular organismal development
GO:0019215 intermediate filament binding
GO:0030057 desmosome
GO:0030280 structural constituent of epidermis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Plakophilin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates
Western Blotting
Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB