Placental Alkaline Phosphatase antibody

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Mouse anti Placental Alkaline Phosphatase

Placental alkaline phosphatase antibody recognizes placental alkaline phosphatase, also known as alkaline phosphatase placental type, Regan isozyme, alkaline phosphomonoesterase or glycerophosphatase.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00669 WB 100 µl
Mouse anti Human placental alkaline phosphatase antibody recognizes placental alkaline phosphatase, also known as alkaline phosphatase placental type, Regan isozyme, alkaline phosphomonoesterase and glycerophosphatase.

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, ALPP is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized (provided by RefSeq, Jul 2008).

Mouse anti Human placental alkaline phosphatase antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti placental alkaline phosphatase antibody detects a band of approximately 70 kDa in human placental tissue
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length recombinant human placental alkaline phosphatase
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P05187 Related reagents
Entrez Gene
ALPP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004035 alkaline phosphatase activity
GO:0016021 integral to membrane
GO:0046872 metal ion binding
GO:0009986 cell surface
GO:0031225 anchored to membrane
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Placental Alkaline Phosphatase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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