Placental Alkaline Phosphatase antibody
Mouse anti Placental Alkaline Phosphatase
Placental alkaline phosphatase antibody recognizes placental alkaline phosphatase, also known as alkaline phosphatase placental type, Regan isozyme, alkaline phosphomonoesterase or glycerophosphatase.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00669 | WB | 100 µl |
Mouse anti Human placental alkaline phosphatase antibody recognizes placental alkaline phosphatase, also known as alkaline phosphatase placental type, Regan isozyme, alkaline phosphomonoesterase and glycerophosphatase.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, ALPP is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized (provided by RefSeq, Jul 2008).
Mouse anti Human placental alkaline phosphatase antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, ALPP is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized (provided by RefSeq, Jul 2008).
Mouse anti Human placental alkaline phosphatase antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti placental alkaline phosphatase antibody detects a band of approximately 70 kDa in human placental tissue
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Full length recombinant human placental alkaline phosphatase
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P05187 Related reagents
- Entrez Gene
- ALPP Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0004035 alkaline phosphatase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0009986 cell surface
- GO:0031225 anchored to membrane
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Placental Alkaline Phosphatase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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