PINK1 antibody | AbD11408

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Human anti Human PINK1

Product Type
Monoclonal Antibody
Clone
AbD11408
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA150A C P* WB 50 µg
HCA150 C P* WB 0.1 mg
Human anti Human PINK1 antibody, clone AbD11408 recognizes human PTEN-induced putative kinase protein 1 (PINK1), a 581 aa single pass membrane protein belonging the serine/threonine protein kinase.

PINK1 protects against mitochondrial dysfunction during cellular stress, possibly by phosphorylating mitochondrial proteins. PINK1 is highly expressed in tissues such as the heart, skeletal muscle and testis. Lower expression levels are detected in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.

Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson's disease 6 (PARK6).

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli.. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Pack Size: 50 µg
Reconstitute with 0.5 ml distilled water
Pack Size: 0.1 mg
Reconstitute with 1.0 ml distilled water
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide corresponding to amino acids 321 – 511 of human PINK1
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q9BXM7 Related reagents
Entrez Gene
PINK1 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0000422 mitochondrion degradation
GO:0016021 integral to membrane
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0006950 response to stress
GO:0007243 intracellular protein kinase cascade
GO:0008219 cell death
GO:0010857 calcium-dependent protein kinase activity
GO:0018105 peptidyl-serine phosphorylation
GO:0031396 regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043254 regulation of protein complex assembly
GO:0055131 C3HC4-type RING finger domain binding
GO:0090200 positive regulation of release of cytochrome c from mitochondria
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Regulatory
For research purposes only

Applications of PINK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1 1/50
Western Blotting 2 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Histology Positive Control Tissue
Human testis
Western Blotting
HCA150 detects a band of approximately 70 kDa in human brain cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti V5-Tag MCA1360 1 mg C E F IF IP R WB
Mouse anti V5-Tag:Alk. Phos. MCA1360A 0.1 mg E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for PINK1 antibody

  1. Marzetti, E. et al. (2016) Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia.
    Exp Gerontol. Nov 12. pii: S0531-5565(16)30259-5. [Epub ahead of print]
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