PI-3 Kinase Subunit Gamma antibody | OTI6D1

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Anti PI-3 Kinase Subunit Gamma Antibody, clone OTI6D1 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PI-3 kinase subunit gamma antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 120 second exposure. Arrow points to PI-3 kinase subunit gamma (molecular weight 126 kDa)

Mouse anti PI-3 Kinase Subunit Gamma

PI-3 kinase subunit gamma antibody recognizes the phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, also known as 1-phosphatidylinositol 3-kinase, PI3-kinase subunit gamma, or phosphatidylinositol 3 kinase gamma.

PI-3 Kinase Subunit Gamma Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI6D1
Isotype: IgG2a

Product Code	Applications	Pack Size
VMA00277	WB	100 µl
VMA00277KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human PI-3 kinase subunit gamma antibody recognizes the phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, also known as 1-phosphatidylinositol 3-kinase, phosphatidylinositol 3 kinase gamma, p110 gamma, phosphatidylinositol 3-kinase catalytic 110-kD gamma, phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, phosphoinositide-3-kinase gamma catalytic subunit, ptdIns-3-kinase subunit gamma, ptdIns-3-kinase subunit p110-gamma and serine/threonine protein kinase PIK3CG.

A product of the PIK3CG gene, PI-3 kinase subunit gamma belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for PIK3CG (provided by RefSeq, Sep 2013).

Mouse anti Human PI-3 kinase subunit gamma isoform antibody detects a band of 126 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human PI-3 kinase subunit gamma (NP_002640) produced in HEK293T cells

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P48736 Related reagents
Entrez Gene: PIK3CG Related reagents
GO Terms: GO:0005515 protein binding; GO:0005524 ATP binding; GO:0005942 phosphatidylinositol 3-kinase complex; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0016303 1-phosphatidylinositol-3-kinase activity; GO:0046934 phosphatidylinositol-4,5-bisphosphate 3-kinase activity; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PI-3 Kinase Subunit Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti PI-3 Kinase subunit gamma detects a band of approximately 126 kDa in K562 cell lysates
Western Blotting: Anti PI-3 Kinase subunit gamma detects a band of approximately 126 kDa in K562 cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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