PI-3 Kinase Regulatory Subunit 1 antibody | D03/2G12-4

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Mouse anti PI-3 Kinase Regulatory Subunit 1

PI-3 kinase regulatory subunit 1 antibody recognizes PIK3R1, also known as p85, AGM7, GRB1, IMD36 or phosphatidylinositol 3-kinase regulatory subunit alpha.

PI-3 Kinase Regulatory Subunit 1 Antibody with Control Lysate

PI-3 kinase regulatory subunit 1 antibody recognizes PIK3R1, also known as p85, AGM7, GRB1, IMD36 or phosphatidylinositol 3-kinase regulatory subunit alpha.

Product Type
PrecisionAb™ Monoclonal
Clone
D03/2G12-4
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00609 WB 100 µl
VMA00609KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PI-3 kinase regulatory subunit 1 antibody recognizes PI-3 kinase regulatory subunit 1 (PIK3R1), also known as p85, AGM7, GRB1, IMD36 or phosphatidylinositol 3-kinase regulatory subunit alpha.

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kDa catalytic subunit and a regulatory subunit of either 85, 55 or 50 kDa. This gene encodes the 85 kDa regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms (provided by RefSeq, Jun 2011).

Mouse anti Human PI-3 kinase regulatory subunit 1 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti PI-3 kinase regulatory subunit 1 antibody detects a band of approximately 85 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human PI-3 kinase regulatory subunit 1 (aa 429-724)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P27986 Related reagents
Entrez Gene
PIK3R1 Related reagents
GO Terms
GO:0043125 ErbB-3 class receptor binding
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005545 1-phosphatidylinositol binding
GO:0005943 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0046326 positive regulation of glucose import
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0035014 phosphatidylinositol 3-kinase regulator activity
GO:0043559 insulin binding
GO:0043560 insulin receptor substrate binding
GO:0044419 interspecies interaction between organisms
GO:0046854 phosphatidylinositol phosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
GO:0060396 growth hormone receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PI-3 Kinase Regulatory Subunit 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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