Phosphoserine Aminotransferase antibody

100% Secure


Mouse anti Phosphoserine Aminotransferase

Phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein or phosphohydroxythreonine aminotransferase. This antibody has been extensively validated for western blotting using whole cell lysates.

Phosphoserine Aminotransferase Antibody with Control Lysate

Phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein or phosphohydroxythreonine aminotransferase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00338 WB 100 µl
VMA00338KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein and phosphohydroxythreonine aminotransferase.

Encoded by the PSAT1 gene, phosphoserine aminotransferase is a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. Decreased expression of this protein may be associated with schizophrenia. Mutations in PSAT1 are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of PSAT1 have been defined on chromosomes 1, 3, and 8 (provided by RefSeq, Jul 2013).

Mouse anti Human phosphoserine aminotransferase antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti phosphoserine aminotransferase detects a band of approximately 41 kDa in A549 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human phosphoserine aminotransferase
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9Y617 Related reagents
Entrez Gene
PSAT1 Related reagents
GO Terms
GO:0004648 O-phospho-L-serine:2-oxoglutarate aminotransferase activity
GO:0006564 L-serine biosynthetic process
GO:0008615 pyridoxine biosynthetic process
GO:0030170 pyridoxal phosphate binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Phosphoserine Aminotransferase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted