Phosphoserine Aminotransferase antibody
Mouse anti Phosphoserine Aminotransferase
Phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein or phosphohydroxythreonine aminotransferase. This antibody has been extensively validated for western blotting using whole cell lysates.
Phosphoserine Aminotransferase Antibody with Control Lysate
Phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein or phosphohydroxythreonine aminotransferase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00338 | WB | 100 µl | ||||
| VMA00338KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human phosphoserine aminotransferase antibody recognizes phosphoserine aminotransferase, also known as endometrial progesterone-induced protein and phosphohydroxythreonine aminotransferase.
Encoded by the PSAT1 gene, phosphoserine aminotransferase is a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. Decreased expression of this protein may be associated with schizophrenia. Mutations in PSAT1 are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of PSAT1 have been defined on chromosomes 1, 3, and 8 (provided by RefSeq, Jul 2013).
Mouse anti Human phosphoserine aminotransferase antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the PSAT1 gene, phosphoserine aminotransferase is a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. Decreased expression of this protein may be associated with schizophrenia. Mutations in PSAT1 are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of PSAT1 have been defined on chromosomes 1, 3, and 8 (provided by RefSeq, Jul 2013).
Mouse anti Human phosphoserine aminotransferase antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti phosphoserine aminotransferase detects a band of approximately 41 kDa in A549 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human phosphoserine aminotransferase
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A549 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9Y617 Related reagents
- Entrez Gene
- PSAT1 Related reagents
- GO Terms
- GO:0004648 O-phospho-L-serine:2-oxoglutarate aminotransferase activity
- GO:0006564 L-serine biosynthetic process
- GO:0008615 pyridoxine biosynthetic process
- GO:0030170 pyridoxal phosphate binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Phosphoserine Aminotransferase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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