Phenylalanine-4-Hydroxylase antibody | AbD12257
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WB ResetHuman anti Human Phenylalanine-4-Hydroxylase
- Product Type
- Monoclonal Antibody
- Clone
- AbD12257
- Isotype
- HuCAL Fab bivalent
- Specificity
- Phenylalanine-4-Hydroxylase
Antibody Quality and 
Unrivaled 
| Human anti Human Phenylalanine-4-Hydroxylase antibody, clone AbD12257 recognizes human phenylalanine-4-hydroxylase (PAH), a 51 kDa homotetrameric protein expressed abundantly in the liver. PAH is an essential enzyme in the metabolic pathway where it is responsible for removing excess phenylalanine by converting it to tyrosine. Defects in the PAH gene, which reduce PAH activity, lead to phenylketonuria (PKU) , a disorder resulting in mental retardation. |
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- StrepTactin affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal.
- Immunogen
- Recombinant human phenylalanine-4-hydroxylase fusion protein with MBP.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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- RRID
- AB_10675606
- UniProt
- P00439
- Entrez Gene
- PAH
- GO Terms
- GO:0042136 neurotransmitter biosynthetic process
- GO:0004505 phenylalanine 4-monooxygenase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006559 L-phenylalanine catabolic process
- GO:0042423 catecholamine biosynthetic process
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