Phenylalanine-4-Hydroxylase antibody | AbD12257

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Anti Human Phenylalanine-4-Hydroxylase Antibody, clone AbD12257 gallery image 1 — COS7 lysate (non-transfected, no PAH, A); A549 lysate (non-transfected, no PAH, B); HEPG2 lysate (non-transfected, endogenous PAH, C); HEK293 lysate (PAH transfected,non stable PAH mutant, D); HEK293 lysate (PAH transfected, wild type PAH, E); Jurkat lysate (non-transfected, no PAH, F) and COS7 lysate (PAH transfected, wild type PAH, G) probed with Human anti Phenylalanine-4-Hydroxylase (HCA141). Image kindly provided by Dr. med. Søren W. Gersting, Ludwig-Maximilians-University Munich

Human anti Human Phenylalanine-4-Hydroxylase

Product Type: Monoclonal Antibody
Clone: AbD12257
Isotype: HuCAL Fab bivalent

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
HCA141	WB			0.1 mg

Human anti Human Phenylalanine-4-Hydroxylase antibody, clone AbD12257 recognizes human phenylalanine-4-hydroxylase (PAH), a 51 kDa homotetrameric protein expressed abundantly in the liver.

PAH is an essential enzyme in the metabolic pathway where it is responsible for removing excess phenylalanine by converting it to tyrosine. Defects in the PAH gene, which reduce PAH activity, lead to phenylketonuria (PKU) , a disorder resulting in mental retardation.

Product Details

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution: Reconstitute with 1.0 ml distilled water
Preparation: StrepTactin affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Recombinant human phenylalanine-4-hydroxylase fusion protein with MBP.
Approx. Protein Concentrations: Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution

More Information

UniProt: P00439 Related reagents
Entrez Gene: PAH Related reagents
GO Terms: GO:0042136 neurotransmitter biosynthetic process; GO:0004505 phenylalanine 4-monooxygenase activity; GO:0005829 cytosol; GO:0055114 oxidation-reduction process; GO:0006559 L-phenylalanine catabolic process; GO:0042423 catecholamine biosynthetic process
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Regulatory: For research purposes only

Applications of Phenylalanine-4-Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Mouse anti c-Myc:Alk.Phos.	MCA2200A	0.1 mg	C E P WB*
Mouse anti c-Myc:Alexa Fluor® 488	MCA2200A488	100 Tests/1ml	F*
Mouse anti c-Myc:Alexa Fluor® 647	MCA2200A647	100 Tests/1ml	F*
Mouse anti c-Myc:Biotin	MCA2200B	0.1 mg	C E P WB*
Mouse anti c-Myc:FITC	MCA2200F	0.1 mg	F*
Mouse anti c-Myc:HRP	MCA2200P	0.1 mg	C E P WB*
Mouse anti c-Myc:RPE	MCA2200PE	100 Tests	WB
Mouse anti Strep-Tag Classic:HRP	MCA2489P	75 µl	E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
HuCAL Fab-dHLX-MSx2 Negative Control	HCA102	0.1 mg	C E F P WB

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