Phenylalanine-4-Hydroxylase antibody | AbD12257

Human anti Human Phenylalanine-4-Hydroxylase

Product Type
Monoclonal Antibody
Clone
AbD12257
Isotype
HuCAL Fab bivalent
Specificity
Phenylalanine-4-Hydroxylase

Product Code Applications Pack Size List Price Your Price Qty
HCA141
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SDS Safety Datasheet SDS
WB 0.1 mg loader
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Human anti Human Phenylalanine-4-Hydroxylase antibody, clone AbD12257 recognizes human phenylalanine-4-hydroxylase (PAH), a 51 kDa homotetrameric protein expressed abundantly in the liver.

PAH is an essential enzyme in the metabolic pathway where it is responsible for removing excess phenylalanine by converting it to tyrosine. Defects in the PAH gene, which reduce PAH activity, lead to phenylketonuria (PKU) , a disorder resulting in mental retardation.

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
StrepTactin affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Recombinant human phenylalanine-4-hydroxylase fusion protein with MBP.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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RRID
AB_10675606
UniProt
P00439
Entrez Gene
PAH
GO Terms
GO:0042136 neurotransmitter biosynthetic process
GO:0004505 phenylalanine 4-monooxygenase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006559 L-phenylalanine catabolic process
GO:0042423 catecholamine biosynthetic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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