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Phenylalanine-4-Hydroxylase antibody | AbD12257

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Anti Human Phenylalanine-4-Hydroxylase Antibody, clone AbD12257 gallery image 1
COS7 lysate (non-transfected, no PAH, A); A549 lysate (non-transfected, no PAH, B); HEPG2 lysate (non-transfected, endogenous PAH, C); HEK293 lysate (PAH transfected,non stable PAH mutant, D); HEK293 lysate (PAH transfected, wild type PAH, E); Jurkat lysate (non-transfected, no PAH, F) and COS7 lysate (PAH transfected, wild type PAH, G) probed with Human anti Phenylalanine-4-Hydroxylase (HCA141). Image kindly provided by Dr. med. Søren W. Gersting, Ludwig-Maximilians-University Munich

Human anti Human Phenylalanine-4-Hydroxylase

Product Type
Monoclonal Antibody
Clone
AbD12257
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA141
Datasheet
WB
MSDS
0.1 mg loader

Human anti Human Phenylalanine-4-Hydroxylase antibody, clone AbD12257 recognizes human phenylalanine-4-hydroxylase (PAH), a 51 kDa homotetrameric protein expressed abundantly in the liver.

PAH is an essential enzyme in the metabolic pathway where it is responsible for removing excess phenylalanine by converting it to tyrosine. Defects in the PAH gene, which reduce PAH activity, lead to phenylketonuria (PKU) , a disorder resulting in mental retardation.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant human phenylalanine-4-hydroxylase fusion protein with MBP.
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution

More Information

UniProt
P00439 Related reagents
Entrez Gene
PAH Related reagents
GO Terms
GO:0042136 neurotransmitter biosynthetic process
GO:0004505 phenylalanine 4-monooxygenase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006559 L-phenylalanine catabolic process
GO:0042423 catecholamine biosynthetic process
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Regulatory
For research purposes only

Applications of Phenylalanine-4-Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti c-Myc:Alk.Phos. MCA2200A C E P WB* 0.1 mg
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 F* 100 Tests/1ml loader
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Mouse anti c-Myc:Biotin MCA2200B C E P WB* 0.1 mg loader
Mouse anti c-Myc:FITC MCA2200F F* 0.1 mg loader
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
Mouse anti c-Myc:RPE MCA2200PE WB 100 Tests loader
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-dHLX-MSx2 Negative Control HCA102 E 0.1 mg loader
Request a different product with the Phenylalanine-4-Hydroxylase specificity