PGRMC1 antibody | CD02/1A2
Mouse anti PGRMC1 antibody recognizes membrane-associated progesterone receptor component 1, also known as mPR, Dap1 and IZA. PGRMC1 is a component of the membrane associated progesterone receptor (MAPR) and has a wide range of functions including vesicle trafficking, regulation of sterol synthesis, regulation of entry into the G0 stage of the cell cycle, invasive growth, and hypoxic biology (Cahill 2017). PGRMC1 has been shown to localize at various subcellular compartments including endoplasmic reticulum, Golgi apparatus, plasma membrane, inner acrosomal membrane, nucleus, nucleolus, and mitochondria (Kim et al. 2019). PGRMC1 appears to be involved with cancers, particularly those of the female reproductive tract, and with central nervous system disorders, such as Alzheimer's Disease (Cahill et al. 2016). PGRMC1 represents a marker of proliferation for many types of cancer cell.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PGRMC1 detects a band of approximately 24 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 44-195 of human PGRMC1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O00264
- Entrez Gene
- PGRMC1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005496 steroid binding
- GO:0005730 nucleolus
- GO:0005789 endoplasmic reticulum membrane
- GO:0005792 microsome
- GO:0009986 cell surface
- GO:0020037 heme binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PGRMC1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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