PGA3 antibody | AbD10785

100% Secure


Human anti Human PGA3

Product Type
Monoclonal Antibody
Clone
AbD10785
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA196 E WB 0.1 mg
Human anti Human PGA3 antibody, clone AbD10785 recognizes human PGA3, also known as Pepsin A-3 and Pepsinogen-3, a secreted enzyme belonging to the peptidase A1 family.

PGA3 is secreted by the gastric mucosa of mammals.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Pepsin N1 fusion protein
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P0DJD8 Related reagents
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of PGA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product is suitable for use in indirect ELISA applications
Western Blotting
Clone AbD10785 detects a band of approximately 47 kDa from human gastric fluid samples

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB