Peroxiredoxin 5 antibody

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Mouse anti Peroxiredoxin 5

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00582
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human peroxiredoxin 5 antibody recognizes peroxiredoxin 5, mitochondrial, also known as Alu co-repressor 1, epididymis secretory protein Li 55, peroxisomal antioxidant enzyme or thioredoxin reductase.

The PRDX5 gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. PRDX5 uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for PRDX5 (provided by RefSeq, Jul 2008).

Mouse anti Human peroxiredoxin 5 antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti peroxiredoxin 5 detects a band of approximately 17 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human peroxiredoxin 5
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P30044 Related reagents
Entrez Gene
PRDX5 Related reagents
GO Terms
GO:0005739 mitochondrion
GO:0004601 peroxidase activity
GO:0005777 peroxisome
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0006954 inflammatory response
GO:0034614 cellular response to reactive oxygen species
GO:0043027 caspase inhibitor activity
GO:0043066 negative regulation of apoptosis
GO:0044445 cytosolic part
GO:0051920 peroxiredoxin activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Peroxiredoxin 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
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