Peroxiredoxin 5 antibody | 3F11

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Mouse anti Peroxiredoxin 5

Product Type
Monoclonal Antibody
Clone
3F11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6026 E IC IP WB 50 µg
Mouse anti peroxiredoxin 5 antibody, clone 3F11, recognizes peroxiredoxin 5 also known as alu co-repressor 1, antioxidant enzyme B166, liver tissue 2D-page spot 71B and thioredoxin peroxidase PMP20. Peroxiredoxin 5 is predominantly localized to the mitochondria but other isoforms have been reported to localize in the cytoplasm and peroxisomes (Knoops et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
IgG fraction prepared by ammonium sulphate precipitation
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.03% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant human peroxiredoxin 5 fragment protein (without mitochondrial leader sequence) purified from E.coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P30044 Related reagents
Entrez Gene
PRDX5 Related reagents
GO Terms
GO:0005739 mitochondrion
GO:0004601 peroxidase activity
GO:0005777 peroxisome
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0006954 inflammatory response
GO:0034614 cellular response to reactive oxygen species
GO:0043027 caspase inhibitor activity
GO:0043066 negative regulation of apoptosis
GO:0044445 cytosolic part
GO:0051920 peroxiredoxin activity
Regulatory
For research purposes only

Applications of Peroxiredoxin 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunocytochemistry 20 ul/ml
Immunoprecipitation 1.0 2.0 ul
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse anti peroxiredoxin 5 recognizes a band of approximately 22 kDa in HeLa, 293T and Jurkat cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F