PDIA3 antibody

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Mouse anti PDIA3

Mouse anti-human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, ER protein 57, ER protein 60, epididymis secretory protein Li 269 or epididymis phospholipase C-alpha.

PDIA3 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00477	WB	100 µl
VMA00477KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human PDIA3 antibody recognizes PDIA3 also known as protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, Grp58, Erp57, ER protein 57, ER protein 60, disulfide isomerase ER-60, endoplasmic reticulum P58, endoplasmic reticulum resident protein 57 or endoplasmic reticulum resident protein 60,

PDIA3 gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates (provided by RefSeq, Jul 2008).

Mouse anti Human PDIA3 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti PDIA3 detects a band of approximately 57 kDa in HepG2 cell lysate
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant protein of human PDIA3
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P30101 Related reagents
Entrez Gene: PDIA3 Related reagents
GO Terms: GO:0005515 protein binding; GO:0004197 cysteine-type endopeptidase activity; GO:0006457 protein folding; GO:0003756 protein disulfide isomerase activity; GO:0006606 protein import into nucleus; GO:0004629 phospholipase C activity; GO:0007165 signal transduction; GO:0005788 endoplasmic reticulum lumen; GO:0045454 cell redox homeostasis; GO:0006621 protein retention in ER lumen; GO:0018279 protein N-linked glycosylation via asparagine; GO:0042470 melanosome; GO:0043687 post-translational protein modification
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PDIA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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