PDIA3 antibody

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Mouse anti PDIA3

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00477
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human PDIA3 antibody recognizes PDIA3 also known as protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, Grp58, Erp57, ER protein 57, ER protein 60, disulfide isomerase ER-60, endoplasmic reticulum P58, endoplasmic reticulum resident protein 57 or endoplasmic reticulum resident protein 60,

PDIA3 gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates (provided by RefSeq, Jul 2008).

Mouse anti Human PDIA3 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PDIA3 detects a band of approximately 57 kDa in HepG2 cell lysate
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein of human PDIA3

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P30101 Related reagents
Entrez Gene
PDIA3 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0006457 protein folding
GO:0003756 protein disulfide isomerase activity
GO:0006606 protein import into nucleus
GO:0004629 phospholipase C activity
GO:0007165 signal transduction
GO:0005788 endoplasmic reticulum lumen
GO:0045454 cell redox homeostasis
GO:0006621 protein retention in ER lumen
GO:0018279 protein N-linked glycosylation via asparagine
GO:0042470 melanosome
GO:0043687 post-translational protein modification
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PDIA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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