PDIA1 antibody

Mouse anti PDIA1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
PDIA1

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VMA00488
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Mouse anti Human PDIA1 antibody recognizes PDIA1, also known as cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, p55, procollagen-proline, 2-oxoglutarate 4-dioxygenase beta polypeptide or thyroid hormone-binding protein p55.

The P4HB gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (provided by RefSeq, Jul 2008).

Mouse anti Human PDIA1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PDIA1 detects a band of approximately 57 kDa in HeLa cell lysate.
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein of human PDIA1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P07237
Entrez Gene
P4HB
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003756 protein disulfide isomerase activity
GO:0005576 extracellular region
GO:0004656 procollagen-proline 4-dioxygenase activity
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0045454 cell redox homeostasis
GO:0006629 lipid metabolic process
GO:0009986 cell surface
GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
GO:0042157 lipoprotein metabolic process
GO:0042470 melanosome
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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