PDIA1 antibody

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Anti PDIA1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PDIA1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to PDIA1 (molecular weight 57 kDa).

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Mouse anti PDIA1

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: PDIA1

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Mouse anti Human PDIA1 antibody recognizes PDIA1, also known as cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, p55, procollagen-proline, 2-oxoglutarate 4-dioxygenase beta polypeptide or thyroid hormone-binding protein p55.

The P4HB gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (provided by RefSeq, Jul 2008).

Mouse anti Human PDIA1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PDIA1 detects a band of approximately 57 kDa in HeLa cell lysate.
Product Form: Purified IgG - liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant protein of human PDIA1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P07237
Entrez Gene: P4HB
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0003756 protein disulfide isomerase activity; GO:0005576 extracellular region; GO:0004656 procollagen-proline 4-dioxygenase activity; GO:0005788 endoplasmic reticulum lumen; GO:0005793 ER-Golgi intermediate compartment; GO:0045454 cell redox homeostasis; GO:0006629 lipid metabolic process; View More GO Terms; GO:0009986 cell surface; GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline; GO:0042157 lipoprotein metabolic process; GO:0042470 melanosome