PDIA1 antibody
Mouse anti Human PDIA1 antibody recognizes PDIA1, also known as cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, p55, procollagen-proline, 2-oxoglutarate 4-dioxygenase beta polypeptide or thyroid hormone-binding protein p55.
The P4HB gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (provided by RefSeq, Jul 2008).
Mouse anti Human PDIA1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The P4HB gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (provided by RefSeq, Jul 2008).
Mouse anti Human PDIA1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PDIA1 detects a band of approximately 57 kDa in HeLa cell lysate
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein of human PDIA1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07237
- Entrez Gene
- P4HB
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0003756 protein disulfide isomerase activity
- GO:0005576 extracellular region
- GO:0004656 procollagen-proline 4-dioxygenase activity
- GO:0005788 endoplasmic reticulum lumen
- GO:0005793 ER-Golgi intermediate compartment
- GO:0045454 cell redox homeostasis
- GO:0006629 lipid metabolic process
- GO:0009986 cell surface
- GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
- GO:0042157 lipoprotein metabolic process
- GO:0042470 melanosome
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PDIA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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