The P4HB gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (provided by RefSeq, Jul 2008).
Mouse anti Human PDIA1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti PDIA1 detects a band of approximately 57 kDa in HeLa cell lysate
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant protein of human PDIA1
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P07237 Related reagents
- Entrez Gene
- P4HB Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0003756 protein disulfide isomerase activity
- GO:0005576 extracellular region
- GO:0004656 procollagen-proline 4-dioxygenase activity
- GO:0005788 endoplasmic reticulum lumen
- GO:0005793 ER-Golgi intermediate compartment
- GO:0045454 cell redox homeostasis
- GO:0006629 lipid metabolic process
- GO:0009986 cell surface
- GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
- GO:0042157 lipoprotein metabolic process
- GO:0042470 melanosome
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of PDIA1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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