p70S6K antibody | 1E12

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Anti Human p70S6K Antibody, clone 1E12 thumbnail image 1

Anti Human p70S6K Antibody, clone 1E12 thumbnail image 2

Anti Human p70S6K Antibody, clone 1E12 thumbnail image 3

Anti Human p70S6K Antibody, clone 1E12 thumbnail image 4

Anti Human p70S6K Antibody, clone 1E12 gallery image 1 — Western blot analysis of p70S6K expression in HeLa cell lysate using preservative free Mouse anti Human p70S6K: (**MCA3430Z**).

Anti Human p70S6K Antibody, clone 1E12 gallery image 2 — Immunofluorescence showing p70S6K in HeLa cells using preservative free Mouse anti Human p70S6K (**MCA3430Z**).

Anti Human p70S6K Antibody, clone 1E12 gallery image 3 — Staining of paraffin embedded human placenta with preservative free Mouse anti Human p70S6K:(**MCA3430Z**).

Anti Human p70S6K Antibody, clone 1E12 gallery image 4 — Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human p70S6K antibody (**MCA3430Z**) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence.

Mouse anti Human p70S6K:Preservative Free

Product Type: Monoclonal Antibody
Clone: 1E12
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA3430Z	IF P* WB			0.1 mg

Mouse anti Human p70S6K antibody, clone 1E12 recognizes human p70S6K, also known as Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A or p70 ribosomal S6 kinase alpha. p70S6K is a 525 amino acid ~70 kDa Serine/threonine-protein kinase acting to promote cell division and survival through inhibition of pro-apoptotic activity of (BAD).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant protein corresponding to aa 416 - 526 of human p70S6K encoded by gene RPS6KB1.
Approx. Protein Concentrations: Ig concentration 0.5 mg/ml
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P23443 Related reagents
Entrez Gene: RPS6KB1 Related reagents
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0004672 protein kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005741 mitochondrial outer membrane; GO:0008286 insulin receptor signaling pathway; GO:0019717 synaptosome; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030054 cell junction; GO:0031929 TOR signaling cascade; GO:0043066 negative regulation of apoptosis; GO:0045202 synapse; GO:0045931 positive regulation of mitotic cell cycle; GO:0045948 positive regulation of translational initiation
Regulatory: For research purposes only

Applications of p70S6K antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence	0.1	10 ug/ml
Immunohistology - Paraffin ¹	0.1	10 ug/ml
Western Blotting

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2b:FITC	HCA038F	0.1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for p70S6K antibody

Bodnar, L. et al. (2015) Clinical usefulness of PI3K/Akt/mTOR genotyping in companion with other clinical variables in metastatic renal cell carcinoma patients treated with everolimus in the second and subsequent lines.
Ann Oncol. 26 (7): 1385-9.

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