p53 antibody
Mouse anti Human p53 antibody recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. The antibody binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992).
The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor, involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).
Mouse anti Human p53 antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53 and detects a band of ~53 kDa on HEK293 cell lysates by western blotting under reducing conditions.
The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor, involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).
Mouse anti Human p53 antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53 and detects a band of ~53 kDa on HEK293 cell lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti p53 antibody detects a band of approximately 53 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity African green monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human p53
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04637 Related reagents
- Entrez Gene
- TP53 Related reagents
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of p53 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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