p53 antibody | DO-1

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Mouse anti p53

Product Type
PrecisionAb Monoclonal
Clone
DO-1
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00019
Datasheet
C E IP P* WB
SDS
100 µl loader

Mouse anti Human p53 antibody, clone DO-1 recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. The antibody binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992).

The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor,  involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).

Mouse anti Human p53 antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53 and detects a band of ~53 kDa on HEK293 cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti p53 antibody detects a band of approximately 53 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
Bovine
Cat
Horse
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human p53
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P04637
Entrez Gene
TP53
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of p53 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Histology Positive Control Tissue
Colon or breast carcinoma

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Application Based External Images

Western Blotting

Product Specific References

References for p53 antibody

  1. Vojtčsek, B. et al. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.
    J Immunol Methods. 151 (1-2): 237-44.
  2. Levesque, M.A. et al. (1995) Time-resolved immunofluorometric assay of p53 protein.
    Clin Chem. 41 (12 Pt 1): 1720-9.
  3. Hietanen, S. et al. (2000) Activation of p53 in cervical carcinoma cells by small molecules.
    Proc Natl Acad Sci U S A. 97 (15): 8501-6.
  4. Carvalho, T. et al. (2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.
    Vet Pathol. 46 (2): 211-21.
  5. Bergman, L.M. et al. (2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.
    Mol Cell Biol. 29 (16): 4539-51.
  6. Phelps, M. et al. (2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.
    Cancer Res. 63 (10): 2616-23.
  7. Phillips, A. et al. (2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.
    J Biol Chem. 285 (38): 29111-27.
  8. Novello, C. et al. (2014) p53-dependent activation of microRNA-34a in response to etoposide-induced DNA damage in osteosarcoma cell lines not impaired by dominant negative p53 expression.
    PLoS One. 9 (12): e114757.

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