p53 antibody | DO-1
The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor, involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).
Mouse anti Human p53 antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53 and detects a band of ~53 kDa on HEK293 cell lysates by western blotting under reducing conditions.
- Target Species
- Western Blotting
- Anti p53 antibody detects a band of approximately 53 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity African green monkey Bovine Cat Horse Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant human p53
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of p53 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
- Histology Positive Control Tissue
- Colon or breast carcinoma
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
Product Specific References
References for p53 antibody
Vojtčsek, B. et al. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.
J Immunol Methods. 151 (1-2): 237-44.
Levesque, M.A. et al. (1995) Time-resolved immunofluorometric assay of p53 protein.
Clin Chem. 41 (12 Pt 1): 1720-9.
Hietanen, S. et al. (2000) Activation of p53 in cervical carcinoma cells by small molecules.
Proc Natl Acad Sci U S A. 97 (15): 8501-6.
Carvalho, T. et al. (2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.
Vet Pathol. 46 (2): 211-21.
Bergman, L.M. et al. (2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.
Mol Cell Biol. 29 (16): 4539-51.
Phelps, M. et al. (2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.
Cancer Res. 63 (10): 2616-23.
Phillips, A. et al. (2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.
J Biol Chem. 285 (38): 29111-27.
Novello, C. et al. (2014) p53-dependent activation of microRNA-34a in response to etoposide-induced DNA damage in osteosarcoma cell lines not impaired by dominant negative p53 expression.
PLoS One. 9 (12): e114757.
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