p300 antibody | NM11
Formaldehyde fixed HeLa cells were stained with MCA6390 at a dilution of 0.5 ug/ml.
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IF ResetMouse anti Human p300
- Product Type
- Monoclonal Antibody
- Clone
- NM11
- Isotype
- IgG1
- Specificity
- p300
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| Mouse anti Human p300 antibody, clone NM11 recognizes histone acetyltransferase p300, also known as E1A-associated protein p300, or EP300. p300 is a histone acetyltransferase enzyme that regulates the transcription of genes via chromatin remodeling. It is crucial for the regulation of cell proliferation, cell division, cell differentiation and the prevention of the growth of cancerous tumors (Ogryzko et al. 1996) (Pao et al. 2000). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- Full-length p300 purified from human 293 cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Chromatin Immunoprecipitation |  |
5 µg per ChIP | |
| Immunofluorescence | |
0.5 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- Q09472
- Entrez Gene
- EP300
- GO Terms
- GO:0001666 response to hypoxia
- GO:0003713 transcription coactivator activity
- GO:0006915 apoptosis
- GO:0004402 histone acetyltransferase activity
- GO:0005813 centrosome
- GO:0007049 cell cycle
- GO:0008013 beta-catenin binding
- GO:0008270 zinc ion binding
- GO:0018076 N-terminal peptidyl-lysine acetylation
- View More GO Terms
- GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
- GO:0043967 histone H4 acetylation
- GO:0044419 interspecies interaction between organisms
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0051091 positive regulation of transcription factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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