p27/Kip1 antibody

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Anti p27/Kip1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human p27/Kip1 antibody (**VMA00657**) followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to p27/Kip1 (molecular weight 27 kDa).

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Mouse anti p27/Kip1

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: p27/Kip1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00657 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human p27/Kip1 antibody recognizes cyclin-dependent kinase inhibitor 1, also known as CDK-interacting protein 1, KIP1, BWS, P27KIP1 or wild-type p53-activated fragment 1.

CDKN1A encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for CDKN1A (provided by RefSeq, Nov 2010).

Mouse anti Human p27/Kip1 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti p27/Kip1 antibody detects a band of approximately 27 kDa in C6 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Full length recombinant human p27/Kip1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P46527
Entrez Gene: CDKN1B
GO Terms: GO:0000079 regulation of cyclin-dependent protein kinase activity; GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005829 cytosol; GO:0004861 cyclin-dependent protein kinase inhibitor activity; GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity; GO:0005654 nucleoplasm; GO:0006917 induction of apoptosis; View More GO Terms; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0007050 cell cycle arrest; GO:0048102 autophagic cell death; GO:0008285 negative regulation of cell proliferation; GO:0019903 protein phosphatase binding; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030308 negative regulation of cell growth; GO:0032582 negative regulation of gene-specific transcription; GO:0048011 nerve growth factor receptor signaling pathway; GO:0071285 cellular response to lithium ion