p15 antibody | DCS-114.1
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Mouse anti Human p15
- Product Type
- Monoclonal Antibody
- Clone
- DCS-114.1
- Isotype
- IgG1
- Specificity
- p15
Antibody Quality and 
Unrivaled 
| Mouse anti Human p15 antibody, clone DCS-114.1 recognises human p15-Ink4b, also known as Cyclin-dependent kinase 4 inhibitor B or Multiple tumor suppressor 2. p15-Ink4b is a 138 amino acid, ~15 kDa cytoplasmic tumor suppressor protein which inhibits the cyclin D dependent kinases cdk4/6. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.08% Sodium Azide - Immunogen
- Full length recombinant p15 His tagged.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunoprecipitation 1 | |
||
| Western Blotting | |
- 1 N.B. Clone DCS114.1 co-precipitates cdk4/cdk6.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for p15 antibody
-
Yoon, G. et al. (2002) Iron chelation-induced senescence-like growth arrest in hepatocyte cell lines: association of transforming growth factor beta1 (TGF-beta1)-mediated p27Kip1 expression.
Biochem J. 366 (Pt 2): 613-21.
- RRID
- AB_2078576
- UniProt
- P42772
- Entrez Gene
- CDKN2B
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0005634 nucleus
- GO:0007050 cell cycle arrest
- GO:0019901 protein kinase binding
- GO:0030219 megakaryocyte differentiation
- View More GO Terms
- GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031670 cellular response to nutrient
- GO:0050680 negative regulation of epithelial cell proliferation
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