p15 antibody | DCS-114.1

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Mouse anti Human p15

Product Type: Monoclonal Antibody
Clone: DCS-114.1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1988	C IP* WB			0.1 mg

Mouse anti Human p15 antibody, clone DCS-114.1 recognises human p15-Ink4b, also known as Cyclin-dependent kinase 4 inhibitor B or Multiple tumor suppressor 2. p15-Ink4b is a 138 amino acid, ~15 kDa cytoplasmic tumor suppressor protein which inhibits the cyclin D dependent kinases cdk4/6.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.08%

Sodium Azide

Immunogen

Full length recombinant p15 His tagged.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P42772 Related reagents
Entrez Gene: CDKN2B Related reagents
GO Terms: GO:0000079 regulation of cyclin-dependent protein kinase activity; GO:0000080 G1 phase of mitotic cell cycle; GO:0000086 G2/M transition of mitotic cell cycle; GO:0005829 cytosol; GO:0004861 cyclin-dependent protein kinase inhibitor activity; GO:0005634 nucleus; GO:0007050 cell cycle arrest; GO:0019901 protein kinase binding; GO:0030219 megakaryocyte differentiation; GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031670 cellular response to nutrient; GO:0050680 negative regulation of epithelial cell proliferation
Regulatory: For research purposes only

Applications of p15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunoprecipitation ¹
Western Blotting

¹ N.B. Clone DCS114.1 co-precipitates cdk4/cdk6.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Western Blotting

Product Specific References

References for p15 antibody

Yoon, G. et al. (2002) Iron chelation-induced senescence-like growth arrest in hepatocyte cell lines: association of transforming growth factor beta1 (TGF-beta1)-mediated p27Kip1 expression.
Biochem J. 366 (Pt 2): 613-21.

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