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p15 antibody | DCS-114.1

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Mouse anti Human p15

Product Type
Monoclonal Antibody
Clone
DCS-114.1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1988
Datasheet
C IP* WB
SDS
0.1 mg loader

Mouse anti Human p15 antibody, clone DCS-114.1 recognises human p15-Ink4b, also known as Cyclin-dependent kinase 4 inhibitor B or Multiple tumor suppressor 2. p15-Ink4b is a 138 amino acid, ~15 kDa cytoplasmic tumor suppressor protein which inhibits the cyclin D dependent kinases cdk4/6.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
Immunogen
Full length recombinant p15 His tagged.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P42772
Entrez Gene
CDKN2B
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005634 nucleus
GO:0007050 cell cycle arrest
GO:0019901 protein kinase binding
GO:0030219 megakaryocyte differentiation
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031670 cellular response to nutrient
GO:0050680 negative regulation of epithelial cell proliferation
Regulatory
For research purposes only

Applications of p15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation 1
Western Blotting
  1. 1 N.B. Clone DCS114.1 co-precipitates cdk4/cdk6.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Western Blotting

Product Specific References

References for p15 antibody

  1. Yoon, G. et al. (2002) Iron chelation-induced senescence-like growth arrest in hepatocyte cell lines: association of transforming growth factor beta1 (TGF-beta1)-mediated p27Kip1 expression.
    Biochem J. 366 (Pt 2): 613-21.

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