p15 antibody | DCS-114.1
No associated image currently available
Mouse anti Human p15 antibody, clone DCS-114.1 recognises human p15-Ink4b, also known as Cyclin-dependent kinase 4 inhibitor B or Multiple tumor suppressor 2. p15-Ink4b is a 138 amino acid, ~15 kDa cytoplasmic tumor suppressor protein which inhibits the cyclin D dependent kinases cdk4/6.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.08% Sodium Azide - Immunogen
- Full length recombinant p15 His tagged.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P42772
- Entrez Gene
- CDKN2B
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0005634 nucleus
- GO:0007050 cell cycle arrest
- GO:0019901 protein kinase binding
- GO:0030219 megakaryocyte differentiation
- GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031670 cellular response to nutrient
- GO:0050680 negative regulation of epithelial cell proliferation
- Regulatory
- For research purposes only
Applications of p15 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunoprecipitation 1 | |||
| Western Blotting |
- 1 N.B. Clone DCS114.1 co-precipitates cdk4/cdk6.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for p15 antibody
-
Yoon, G. et al. (2002) Iron chelation-induced senescence-like growth arrest in hepatocyte cell lines: association of transforming growth factor beta1 (TGF-beta1)-mediated p27Kip1 expression.
Biochem J. 366 (Pt 2): 613-21.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
