p130Cas antibody | OTI3A11

Mouse anti p130Cas

Product Type
PrecisionAb Monoclonal
Clone
OTI3A11
Isotype
IgG1
Format
Purified
Specificity
p130Cas

Product Code Applications Pack Size List Price Your Price Qty
VMA00132
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader
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Mouse anti Human p130Cas antibody, clone OTI3A11 recognizes p130Cas, also known as Cas scaffolding protein family member 1, Crk-associated substrate p130Cas and breast cancer anti-estrogen resistance protein 1.

p130Cas, enclode by the BCAR1 gene, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al. 2006).

Mouse anti Human p130Cas antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti p130Cas detects a band of approximately 130 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human p130Cas (NP_055382) produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Further Reading

  1. Sawada Y et al. (2006) Force sensing by mechanical extension of the Src family kinase substrate p130Cas.
    Cell 127 (5): 1015-26.

UniProt
P56945
Entrez Gene
BCAR1
GO Terms
GO:0001558 regulation of cell growth
GO:0001726 ruffle
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005624 membrane fraction
GO:0005925 focal adhesion
GO:0007015 actin filament organization
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007229 integrin-mediated signaling pathway
GO:0008283 cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0042981 regulation of apoptosis
GO:0016477 cell migration
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0030335 positive regulation of cell migration
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0051301 cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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