p130Cas antibody | OTI3A11

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Anti p130Cas Antibody, clone OTI3A11 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with p130Cas antibody (**VMA00132**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 69 second exposure. Arrow points to p130Cas (molecular weight 130 kDa).

Mouse anti p130Cas

p130Cas antibody recognizes breast cancer anti-estrogen resistance protein 1, also known as Cas scaffolding protein family member 1 or CRK-associated substrate p130Cas. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI3A11
Isotype: IgG1
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00132	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human p130Cas antibody recognizes p130Cas, also known as Cas scaffolding protein family member 1, Crk-associated substrate p130Cas and breast cancer anti-estrogen resistance protein 1.

p130Cas, enclode by the BCAR1 gene, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al. 2006).

Mouse anti Human p130Cas antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti p130Cas detects a band of approximately 130 kDa in MCF7 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant protein of human p130Cas (NP_055382) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P56945 Related reagents
Entrez Gene: BCAR1 Related reagents
GO Terms: GO:0001558 regulation of cell growth; GO:0001726 ruffle; GO:0007596 blood coagulation; GO:0007155 cell adhesion; GO:0005829 cytosol; GO:0004871 signal transducer activity; GO:0005624 membrane fraction; GO:0005925 focal adhesion; GO:0007015 actin filament organization; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0007229 integrin-mediated signaling pathway; GO:0008283 cell proliferation; GO:0008286 insulin receptor signaling pathway; GO:0042981 regulation of apoptosis; GO:0016477 cell migration; GO:0017124 SH3 domain binding; GO:0019901 protein kinase binding; GO:0019903 protein phosphatase binding; GO:0030335 positive regulation of cell migration; GO:0048008 platelet-derived growth factor receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0051301 cell division
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of p130Cas antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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