p130Cas antibody | OTI3A11
Mouse anti p130Cas
p130Cas antibody recognizes breast cancer anti-estrogen resistance protein 1, also known as Cas scaffolding protein family member 1 or CRK-associated substrate p130Cas. This antibody has been extensively validated for western blotting using whole cell lysates.
p130Cas Antibody with Control Lysate
p130Cas antibody recognizes breast cancer anti-estrogen resistance protein 1, also known as Cas scaffolding protein family member 1 or CRK-associated substrate p130Cas. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3A11
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00132 | WB | 100 µl | ||||
| VMA00132KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human p130Cas antibody recognizes p130Cas, also known as Cas scaffolding protein family member 1, Crk-associated substrate p130Cas and breast cancer anti-estrogen resistance protein 1.
p130Cas, enclode by the BCAR1 gene, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al. 2006).
Mouse anti Human p130Cas antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
p130Cas, enclode by the BCAR1 gene, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al. 2006).
Mouse anti Human p130Cas antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti p130Cas detects a band of approximately 130 kDa in MCF7 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant protein of human p130Cas (NP_055382) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P56945 Related reagents
- Entrez Gene
- BCAR1 Related reagents
- GO Terms
- GO:0001558 regulation of cell growth
- GO:0001726 ruffle
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005624 membrane fraction
- GO:0005925 focal adhesion
- GO:0007015 actin filament organization
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007229 integrin-mediated signaling pathway
- GO:0008283 cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0042981 regulation of apoptosis
- GO:0016477 cell migration
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0019903 protein phosphatase binding
- GO:0030335 positive regulation of cell migration
- GO:0048008 platelet-derived growth factor receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of p130Cas antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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