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p130Cas antibody | OTI3A11

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Western Blot Validated
Anti p130Cas Antibody, clone OTI3A11 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with p130Cas antibody (VMA00132) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 69 second exposure. Arrow points to p130Cas (molecular weight 130 kDa).

Mouse anti p130Cas

Product Type
PrecisionAb Monoclonal
Clone
OTI3A11
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00132
Datasheet
WB
SDS
100 µl loader

Mouse anti Human p130Cas antibody recognizes p130Cas, also known as Cas scaffolding protein family member 1, Crk-associated substrate p130Cas and breast cancer anti-estrogen resistance protein 1.

p130Cas, enclode by the BCAR1 gene, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al. 2006).

Mouse anti Human p130Cas antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti p130Cas detects a band of approximately 130 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human p130Cas (NP_055382) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P56945
Entrez Gene
BCAR1
GO Terms
GO:0001558 regulation of cell growth
GO:0001726 ruffle
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005624 membrane fraction
GO:0005925 focal adhesion
GO:0007015 actin filament organization
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007229 integrin-mediated signaling pathway
GO:0008283 cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0042981 regulation of apoptosis
GO:0016477 cell migration
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0030335 positive regulation of cell migration
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of p130Cas antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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