OTUB1 antibody | AB01/3A9

Mouse anti OTUB1

Product Type
PrecisionAb Monoclonal
Clone
AB01/3A9
Isotype
IgG2b
Format
Purified
Specificity
OTUB1

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VMA00919
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IP WB 100 µl loader
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Mouse anti OTUB1 antibody recognizes ubiquitin thioesterase OTUB1, also known as otubain-1 and hOTU1.

OTUB1 is a deubiquitinating enzyme that specifically cleaves Lys-48-linked polyubiquitin chains, controls the stability of multiple proteins and regulates a range of cellular processes. Notably, OTUB1 stabilizes transcription factors including FOXM1 and ER-alpha, and the E2-conjugating enzyme UBE2E1 (Pasupala et al. 2018). High OTUB1 expression has been identified in multiple cancer types, and OTUB1 overexpression has been found to promote metastasis and negatively affect prognosis and quality of life of patients with cancer (Ni et al. 2017). OTUB1 plays a role in the cellular response to DNA damage, and is suggested to protect the brain from secondary damage following intracranial hemorrhage (Xie et al. 2016).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti OTUB1 detects a band of approximately 35 kDa in MCF-7 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-271 of human OTUB1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q96FW1
Entrez Gene
OTUB1
GO Terms
GO:0004843 ubiquitin-specific protease activity
GO:0005737 cytoplasm
GO:0019784 NEDD8-specific protease activity
GO:0043130 ubiquitin binding
GO:0071108 protein K48-linked deubiquitination
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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