Osteoprotegerin antibody | E01-10F11
Filter by Application:
WB ResetMouse anti Human Osteoprotegerin
- Product Type
- Monoclonal Antibody
- Clone
- E01-10F11
- Isotype
- IgG1
- Specificity
- Osteoprotegerin
Antibody Quality and Performance Guarantee
| Mouse anti Human osteoprotegerin, clone E01-10F11, recognizes osteoprotegerin (OPG), a cytokine receptor and member of the tumour necrosis factor (TNF) receptor superfamily. The role of OPG involves it acting as a decoy receptor for the ligand of CD254 and thereby preventing its function in osteoclastogenesis. In addition to this it may act as a decoy receptor for TNF related apoptosis inducing ligand (TRAIL) (Emery et al. 1998 and Schoppet et al. 2002). The combination of these two roles allows OPG to inhibit NFkB’s regulatory effects on inflammation, skeletal, and vascular systems. OPG is highly expressed in a wide range of tissues, including adult heart, lung, liver, prostate, ovary and bone marrow. More recently a role for OPG in cardiovascular disease has emerged and it is now clinically relevant biomarker of vascular health (Bierre, 2013). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant protein corresponding to aa 22-201 of human osteoprotegerin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Emery, J.G. et al. (1998) Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL.
J Biol Chem. 273 (23): 14363-7. -
Schoppet, M. et al. (2004) Localization of osteoprotegerin, tumor necrosis factor-related apoptosis-inducing ligand, and receptor activator of nuclear factor-kappaB ligand in Mönckeberg's sclerosis and atherosclerosis.
J Clin Endocrinol Metab. 89 (8): 4104-12. -
Bjerre, M. (2013) Osteoprotegerin (OPG) as a biomarker for diabetic cardiovascular complications.
Springerplus. 2: 658.
- Synonyms
- TNFRSF11B
- UniProt
- O00300
- Entrez Gene
- TNFRSF11B
- GO Terms
- GO:0001501 skeletal system development
- GO:0006915 apoptosis
- GO:0004872 receptor activity
- GO:0005125 cytokine activity
MCA5970GA
280814
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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