Osteoprotegerin antibody | E01-10F11

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Mouse anti Human Osteoprotegerin

Product Type
Monoclonal Antibody
Clone
E01-10F11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5970GA E WB 0.1 mg
Mouse anti Human osteoprotegerin, clone E01-10F11, recognizes osteoprotegerin (OPG), a cytokine receptor and member of the tumour necrosis factor (TNF) receptor superfamily. The role of OPG involves it acting as a decoy receptor for the ligand of CD254 and thereby preventing its function in osteoclastogenesis. In addition to this it may act as a decoy receptor for TNF related apoptosis inducing ligand (TRAIL) (Emery et al. 1998 and Schoppet et al. 2002). The combination of these two roles allows OPG to inhibit NFkB’s regulatory effects on inflammation, skeletal, and vascular systems.

OPG is highly expressed in a wide range of tissues, including adult heart, lung, liver, prostate, ovary and bone marrow. More recently a role for OPG in cardiovascular disease has emerged and it is now clinically relevant biomarker of vascular health (Bierre, 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to aa 22-201 of human osteoprotegerin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
O00300 Related reagents
Entrez Gene
TNFRSF11B Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0006915 apoptosis
GO:0004872 receptor activity
GO:0005125 cytokine activity
Regulatory
For research purposes only

Applications of Osteoprotegerin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. Emery, J.G. et al. (1998) Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL.
    J Biol Chem. 273 (23): 14363-7.
  2. Schoppet, M. et al. (2004) Localization of osteoprotegerin, tumor necrosis factor-related apoptosis-inducing ligand, and receptor activator of nuclear factor-kappaB ligand in Mönckeberg's sclerosis and atherosclerosis.
    J Clin Endocrinol Metab. 89 (8): 4104-12.
  3. Bjerre, M. (2013) Osteoprotegerin (OPG) as a biomarker for diabetic cardiovascular complications.
    Springerplus. 2: 658.