Osteopontin antibody | F11-4C11

Mouse anti Human Osteopontin

Product Type
Monoclonal Antibody
Clone
F11-4C11
Isotype
IgG1
Specificity
Osteopontin

Product Code Applications Pack Size List Price Your Price Qty
MCA6262GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human osteopontin antibody, clone F11-4C11, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016). OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017). OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016).

The purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
HEK293 derived recombinant human full length osteopontin (Ile17-Asn314)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Osteopontin:Biotin MCA6263B E 0.1 mg loader
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loader
Description Mouse anti Human Osteopontin:Biotin

UniProt
P10451
Entrez Gene
SPP1
GO Terms
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007566 embryo implantation
GO:0031214 biomineral tissue development
GO:0033280 response to vitamin D
GO:0046697 decidualization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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