Osteopontin antibody | F03-4E8
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E ResetMouse anti Human Osteopontin:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- F03-4E8
- Isotype
- IgG1
- Specificity
- Osteopontin
Antibody Quality and Performance Guarantee
| Mouse anti Human osteopontin antibody, clone F03-4E8, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016).
OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017).
OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016). The biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- HEK293 derived recombinant human full length osteopontin (Ile17-Asn314)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human Osteopontin | MCA6262GA | E | 0.1 mg |
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| Description | Mouse anti Human Osteopontin | ||||||
- UniProt
- P10451
- Entrez Gene
- SPP1
- GO Terms
- GO:0007155 cell adhesion
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0007566 embryo implantation
- GO:0031214 biomineral tissue development
- GO:0033280 response to vitamin D
- GO:0046697 decidualization
MCA6263B
100005185
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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