Osteopontin antibody | 1F5

Mouse anti Human Osteopontin:Preservative Free

Product Type
Monoclonal Antibody
Clone
1F5
Isotype
IgG2a
Specificity
Osteopontin

Product Code Applications Pack Size List Price Your Price Qty
MCA5570Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human osteopontin antibody, clone 1F5 recognizes human osteopontin, also known as Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein or Uropontin. Osteopontin is a 298 amino acid ~37 kDa hydroxyapatite binding protein forming part of the mineralized matrix. found in bone and circulating in plasma.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-301 of human osteopontin.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Synonyms
SPP1
RRID
AB_2194834
UniProt
P10451
Entrez Gene
SPP1
GO Terms
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007566 embryo implantation
GO:0031214 biomineral tissue development
GO:0033280 response to vitamin D
GO:0046697 decidualization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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