Osteopontin antibody | 1F5
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WB ResetMouse anti Human Osteopontin:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1F5
- Isotype
- IgG2a
- Specificity
- Osteopontin
Antibody Quality and Performance Guarantee
| Mouse anti Human osteopontin antibody, clone 1F5 recognizes human osteopontin, also known as Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein or Uropontin. Osteopontin is a 298 amino acid ~37 kDa hydroxyapatite binding protein forming part of the mineralized matrix. found in bone and circulating in plasma. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-301 of human osteopontin.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- SPP1
- RRID
- AB_2194834
- UniProt
- P10451
- Entrez Gene
- SPP1
- GO Terms
- GO:0007155 cell adhesion
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0007566 embryo implantation
- GO:0031214 biomineral tissue development
- GO:0033280 response to vitamin D
- GO:0046697 decidualization
MCA5570Z
147892
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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