OCT4 antibody | OTI2B6

Mouse anti OCT4

Product Type
PrecisionAb Monoclonal
Clone
OTI2B6
Isotype
IgG2b
Format
Purified
Specificity
OCT4

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VMA00234
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WB 100 µl loader
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Mouse anti Human OCT4 antibody, clone OTI2B6 recognizes the POU domain, class 5, transcription factor 1, also known as POU domain transcription factor OCT4, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4 and octamer-binding transcription factor 3.

The OCT4 gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of POU5F1 with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq, Mar 2010).

Mouse anti Human OCT4 antibody, clone OTI2B6 detects a band of 45 kDa. The OCT4 antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti OCT4 detects a band of approximately 45 kDa in NTERA-2 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol.
Immunogen
Full length recombinant human OCT4 (NP_002692) produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q01860
Entrez Gene
POU5F1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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