NUMB antibody | OTI3E5

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Anti NUMB Antibody, clone OTI3E5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti NUMB Antibody, clone OTI3E5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NUMB antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to NUMB (molecular weight 72, 74 kDa)

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Mouse anti NUMB

Product Type: PrecisionAb Monoclonal
Clone: OTI3E5
Isotype: IgG1
Format: Purified
Specificity: NUMB

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Mouse anti Human NUMB antibody recognizes the protein numb homolog, also known as h-Numb.

Protein numb homolog is encoded by NUMB gene and plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for NUMB (provided by RefSeq, Jul 2008).

Mouse anti Human NUMB antibody detects bands of 72 kDa and 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti NUMB detects band of approximately 72 kDa and 74 kDa in A549 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.