NUMB antibody | OTI3E5

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Mouse anti NUMB

Product Type
PrecisionAb Monoclonal
Clone
OTI3E5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00262
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human NUMB antibody recognizes the protein numb homolog, also known as h-Numb.

Protein numb homolog is encoded by NUMB gene and plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for NUMB (provided by RefSeq, Jul 2008).

Mouse anti Human NUMB antibody detects bands of 72 kDa and 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti NUMB detects band of approximately 72 kDa and 74 kDa in A549 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P49757 Related reagents
Entrez Gene
NUMB Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NUMB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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