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NUMA1 antibody

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Western Blot Validated
Anti NUMA1 Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with NUMA1 antibody followed by detection with Goat anti Mouse IgM:HRP (1/10,000, 102005102005) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to NUMA1 (molecular weight 280 kDa)

Mouse anti NUMA1

NUMA1 antibody recognizes nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22 or NMP22.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgM
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00690
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human NUMA1 antibody recognizes the nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22, NMP22 and structural nuclear protein.

Mouse anti Human NUMA1 antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti NUMA1 detects a band of approximately 280 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - Liquid
Preparation
Mouse monoclonal antibody purified by PEG precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Recombinant protein of human NUMA1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q14980 Related reagents
Entrez Gene
NUMA1 Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000090 mitotic anaphase
GO:0005515 protein binding
GO:0000922 spindle pole
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005876 spindle microtubule
GO:0006997 nucleus organization
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NUMA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgM:HRP 102005 C E IB 1 ml loader

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