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NUMA1 antibody

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Western Blot Validated
Anti NUMA1 Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with NUMA1 antibody followed by detection with Goat anti Mouse IgM:HRP (1/10,000, 102005102005) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to NUMA1 (molecular weight 280 kDa)

Mouse anti NUMA1

NUMA1 antibody recognizes nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22 or NMP22.

NUMA1 Antibody with Control Lysate

NUMA1 antibody recognizes nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22 or NMP22.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgM
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00690 WB 100 µl
VMA00690KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human NUMA1 antibody recognizes the nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22, NMP22 and structural nuclear protein.

Mouse anti Human NUMA1 antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti NUMA1 detects a band of approximately 280 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - Liquid
Product Form
Purified IgM - Liquid
Preparation
Mouse monoclonal antibody purified by PEG precipitation from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by PEG precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Recombinant protein of human NUMA1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q14980 Related reagents
Entrez Gene
NUMA1 Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000090 mitotic anaphase
GO:0005515 protein binding
GO:0000922 spindle pole
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005876 spindle microtubule
GO:0006997 nucleus organization
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NUMA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgM:HRP 102005 1 ml C E IB
Goat anti Mouse IgM:HRP 102005 1 ml C E IB
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