NUMA1 antibody
Mouse anti NUMA1
NUMA1 antibody recognizes nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22 or NMP22.
NUMA1 Antibody with Control Lysate
NUMA1 antibody recognizes nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22 or NMP22.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgM
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00690 | WB | 100 µl | ||||
| VMA00690KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human NUMA1 antibody recognizes the nuclear mitotic apparatus protein 1, also known as SP-H antigen, nuclear matrix protein-22, NMP22 and structural nuclear protein.
Mouse anti Human NUMA1 antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human NUMA1 antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti NUMA1 detects a band of approximately 280 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - Liquid
- Product Form
- Purified IgM - Liquid
- Preparation
- Mouse monoclonal antibody purified by PEG precipitation from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by PEG precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- Recombinant protein of human NUMA1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14980 Related reagents
- Entrez Gene
- NUMA1 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000090 mitotic anaphase
- GO:0005515 protein binding
- GO:0000922 spindle pole
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005654 nucleoplasm
- GO:0005694 chromosome
- GO:0005876 spindle microtubule
- GO:0006997 nucleus organization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NUMA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgM:HRP | 102005 | 1 ml | C E IB | ||
| Goat anti Mouse IgM:HRP | 102005 | 1 ml | C E IB |
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