Nucleoside Diphosphate Kinase A antibody

Mouse anti Nucleoside Diphosphate Kinase A

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
Nucleoside Diphosphate Kinase A

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VMA00345
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Mouse anti Human nucleoside diphosphate kinase A antibody recognizes nucleoside diphosphate kinase A, also known as NDP kinase A, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1 and tumor metastatic process-associated protein.

The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by NME2) isoforms. Mutations in NME1 have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for NME1. Co-transcription of NME1 and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product (provided by RefSeq, Jul 2008).

Mouse anti Human nucleoside diphosphate kinase A antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti nucleoside diphosphate kinase A detects a band of approximately 17 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human nucleoside diphosphate kinase A
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P15531
Entrez Gene
NME1
GO Terms
GO:0000287 magnesium ion binding
GO:0004536 deoxyribonuclease activity
GO:0004550 nucleoside diphosphate kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0005634 nucleus
GO:0006183 GTP biosynthetic process
GO:0006228 UTP biosynthetic process
GO:0006241 CTP biosynthetic process
GO:0006897 endocytosis
GO:0007399 nervous system development
GO:0008285 negative regulation of cell proliferation
GO:0042802 identical protein binding
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
GO:0042981 regulation of apoptosis
GO:0030154 cell differentiation
GO:0043388 positive regulation of DNA binding
GO:0050679 positive regulation of epithelial cell proliferation